Human IL-17RB Biotinylated Antibody

Name: Human IL-17RB Biotinylated Antibody
Brand: R&D Systems
SKU: BAF1207
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF1207

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human IL‑17 RB Arg18-Gly289 Accession # NP_061195
Applications:	Immunohistochemistry, Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects human IL‑17 RB in Western blots. In Western blots, approximately 35% cross-reactivity with recombinant mouse (rm) IL-17 RB is observed, 5% cross-reactivity with rmIL-17 R is observed, and less than 1% cross-reactivity with recombinant human IL-17 R is observed.

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Applications for Human IL-17RB Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IL‑17 RB Fc Chimera (Catalog # 1207-BR)
Immunohistochemistry	5-15 µg/mL	Immersion fixed paraffin-embedded sections of human kidney

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-17RB

The interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL-17F), are structurally related proteins with a conserved cysteine-knot structure. These proinflammatory cytokines can induce local cytokine productions and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1). Interleukin-17 B Receptor (IL-17 RB), also known as IL-17Rh1, IL-17ER and EVI27, represents the second receptor of the IL-17 family of cytokines to be recognized (2-4). Human IL-17 RB cDNA encodes a 502 amino acid (aa) residue type I membrane protein with a putative 17 aa signal peptide, a 275 aa extracellular domain, a 21 aa transmembrane domain and a 189 aa cytoplasmic tail. By alternative splicing, a secreted variant of IL-17 RB has also been identified (4). Human and mouse IIL-17 RB share 76% aa sequence identity. The human IL-17 RB protein sequence is only 19.2% identical to the human IL-17 R sequence, but the two receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. Three additional type I transmembrane receptors with homology to IL-17 R have been reported, increasing the number of the IL-17 R family members to five (5, 6). By Northern blot analysis, human IL-17 RB is highly expressed in kidneys and liver but is expressed at lower levels in testes, brain, small intestine and other endocrine tissues (2-4). The expression of IL-17 RB is significantly up-regulated under inflammatory conditions. IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17, IL-17C, and IL-17F. Activation of IL-17 RB by its ligands results in the activation of nuclear factor kappa-B (2-4).

Item	Value
References	Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1. Shi Y, et al. (2000) J. Biol. Chem. 275:19167. Lee, J, et al. (2001) J. Biol. Chem. 276:1660. Tian E, et al. (2000) Oncogene 19:2098. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
Long Name	Interleukin 17B Receptor
Entrez Gene IDs	55540 (Human); 50905 (Mouse)
Alternate Names	CRL4IL-17 receptor homolog 1, Cytokine receptor-like 4, EVI27IL-17B receptor, Evi27, IL-17 RB, IL-17 RH1, IL-17 receptor B, IL-17ER, IL-17RB, IL-17Rh1, IL17BRinterleukin 17B receptor, IL17RB, IL17RH1MGC5245, IL17Rh1, Interleukin-17B receptor, cytokine receptor CRL4, interleukin 17 receptor B, interleukin 17 receptor homolog 1, interleukin-17 receptor B

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Human IL-17 RB Biotinylated Antibody

Name: Anonymous

Application: Flow

Sample Tested: Peripheral blood mononuclear cells (PBMCs)

Species: Human

Verified Customer | Posted 01/11/2018

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Human IL-17RB Biotinylated Antibody

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Formulation, Preparation and Storage

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Background: IL-17RB

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Human IL-17 RB Biotinylated Antibody

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