Human IL-10 R alpha Biotinylated Antibody

Name: Human IL-10 R alpha Biotinylated Antibody
Brand: R&D Systems
SKU: BAF274

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF274

Citations (2)

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Species Reactivity:	Human
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑10 R alpha (R&D Systems, Catalog # 274-R1) His22-Asn235 Accession # Q13651
Applications:	Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects human IL-10 R alpha in Western blots. In this format, less than 10% cross-reactivity with recombinant mouse IL‑10 R alpha is observed.

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Applications for Human IL-10 R alpha Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IL‑10 R alpha (Catalog # 274-R1)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-10 R alpha

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), is a potent immunosuppressant of macrophage functions. IL-10 is also a pleiotropic cytokine with multiple immunostimulatory as well as immunosuppressive effects on a variety of other cell types. IL-10 binds specifically and with high affinity to cell-surface receptors. Mouse and human cDNA clones encoding the ligand-binding IL-10 receptor (IL-10 R) have been isolated. The IL-10 R mRNA has been detected in all cell types that are known to respond to IL-10.

Human and mouse IL-10 receptors are structurally related to the IFN-gamma receptor. These receptors are members of the class II subgroup of the cytokine receptor superfamily. The deduced amino acid sequence of human IL-10 R is approximately 60% identical to mouse IL-10 R. Although human IL-10 has cross-species activities and is active on mouse cells, mouse IL-10 is species-specific in its actions and does not bind to the human IL-10 receptor. The human IL-10 R gene has been mapped to chromosome 11q23.3.

Item	Value
Long Name	Interleukin 10 Receptor alpha
Entrez Gene IDs	3587 (Human); 16154 (Mouse); 102127536 (Cynomolgus Monkey)
Alternate Names	CD210 antigen, CDw210a, HIL-10R, IBD28, IL-10 R alpha, IL-10 receptor subunit alpha, IL-10R subunit 1, IL-10R1, IL-10Ra, IL10R alpha, IL10RA, IL10RIL-10R subunit alpha, Interleukin-10 receptor subunit 1, interleukin 10 receptor, alpha, interleukin-10 receptor alpha chain, interleukin-10 receptor subunit alpha

Citations for Human IL-10 R alpha Biotinylated Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-10 R alpha Biotinylated Antibody include:

Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development

Gonzalez RM et al. (2008), Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 7(6):2406-14.
PMID: 18422355
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Corinti S et al. (2001), Regulatory activity of autocrine IL-10 on dendritic cell functions. J. Immunol., 166(7):4312-8.
PMID: 11254683

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Human IL-10 R alpha Biotinylated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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