Detects endogenous human IKK beta in Western blots. In Western blots, this antibody does not cross-react with recombinant human IKK alpha, IKK gamma, or IKK epsilon.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human IKK beta Antibody

Detection of Human IKK beta by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Raji human Burkitt's lymphoma cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human IKK beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4535) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IKK beta at approximately 87 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human IKK beta Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line

Reviewed Applications

Read 1 review rated 5 using AF4535 in the following applications:

ELISA (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IKK beta

I kappaB kinase beta (IKK beta) is also known as IKBKB and IKK2. The classical active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma, phosphorylates and inactivates I kappaB, resulting in the release and nuclear translocation of active NF-kappa B. Like IKK alpha, IKK beta contains kinase, leucine zipper, and helix‑loop‑helix domains. NF-kappa B-inducing kinase (NIK) phosphorylates and activates IKK alpha/IKK beta heterodimers.

Long Name

IkB Kinase beta

Alternate Names

IkBKB, IKK2, NFKBIKB

Entrez Gene IDs

3551 (Human); 16150 (Mouse); 84351 (Rat)

Gene Symbol

IKBKB

UniProt

O14920

Additional IKK beta Products

Product Documents for Human IKK beta Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IKK beta Antibody

For research use only

Citations
Reviews

Human IKK beta Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4535

Key Product Details

Species Reactivity

Validated:

Cited:

Applications