Human IGFBP‑6 Antibody

Name: Human IGFBP‑6 Antibody
Brand: R&D Systems
SKU: MAB8761

Catalog # MAB8761 | R&D Systems, Inc. a Bio-Techne Brand

Citations (4)

Product Datasheet / COA / SDS

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Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA Capture (Matched Antibody Pair), Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 110211

Product Summary for Human IGFBP‑6 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IGFBP-6
Ala25-Gly240
Accession # P24592

Specificity

Detects human IGFBP-6 in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) IGFBP-1, -2, -3, -4, -5, rmIGFBP-3, -5, or -6.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Applications for Human IGFBP‑6 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human IGFBP‑6 (Catalog # 876-B6)

Human IGFBP-6 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human IGFBP‑6 Biotinylated Antibody (Catalog # BAF876)
Standard: Recombinant Human IGFBP-6 Protein, CF (Catalog # 876-B6)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 4 publications using MAB8761 in the following applications:

Antibody Array Development (1 Publication)
IHC-P (1 Publication)
Western Blot (3 Publications)

Formulation, Preparation and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-6

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-transitional modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Human IGFBP-6 cDNA encodes a 240 amino acid (aa) residue precursor protein with a putative 24 aa residue signal peptide that is processed to generate the 216 aa residue mature protein that is O-glycosylated. IGFBP-6 is expressed in ovarian cells, prostatic cells, and fibroblasts. IGFBP-6 is found predominantly in CSF and serum. IGFBP-6 binds preferentially to IGF-II, exhibiting a 2-fold higher affinity for IGF-II than for IGF-I.

References

Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.

Long Name

Insulin-like Growth Factor Binding Protein 6

Alternate Names

IGFBP6

Entrez Gene IDs

3489 (Human); 16012 (Mouse)

Gene Symbol

IGFBP6

UniProt

P24592

Product Documents for Human IGFBP‑6 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IGFBP‑6 Antibody

For research use only

Citations for Human IGFBP‑6 Antibody (4)

Showing 1 - 4 of 4 publications Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human IGFBP‑6 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

M Conese et al. (2020-06-22), Insulin-Like Growth Factor Binding Protein 6 Is Secreted in Extracellular Vesicles upon Hyperthermia and Oxidative Stress in Dendritic Cells But Not in Monocytes Int J Mol Sci, 2020-021(12).
PMID: 32580339
Species: Human
Sample Types: Whole Tissue,Cell Lysates
Applications: Western Blot,IHC-P

Q Chen et al. (2016-10-18), IGFBP6 is a novel nasopharyngeal carcinoma prognostic biomarker Oncotarget, 2016-17(42):68140-68150.
PMID: 27623076
Species: Human
Sample Types: Urine
Applications: Antibody Array Development

Hu H et al. (2009-06-27), Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation, 2009-087(12):1814-20.
PMID: 19543058
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot

Nakamura M et al. (2005-08-05), Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability. Biochem. Biophys. Res. Commun., 2005-0333(3):1011-6.
PMID: 15964556

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