Human IGF-I R/IGF1R Fluorescein-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB391F
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry, Flow Cytometry Control
Label
FITC (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 33255
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IGF-I R
Glu31-Asn932
Accession # P08069
Glu31-Asn932
Accession # P08069
Specificity
Detects human IGF-I R in sandwich immunoassays and Western blots. In sandwich immunoassays, less than 0.15% cross-reactivity or interference was observed with recombinant human (rh) IGF-I, rhIGF-II, rhIL-3 R alpha, rhIL-9 R, and rhTGF-beta RII.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human IGF-I R/IGF1R Fluorescein-conjugated Antibody
Detection of IGF-I R in MCF-7 Human Cell Line by Flow Cytometry.
MCF-7 human breast cancer cell line was stained with Mouse Anti-Human IGF-I R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB391F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human IGF-I R/IGF1R Fluorescein-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: MCF‑7 human breast cancer cell line
Sample: MCF‑7 human breast cancer cell line
Reviewed Applications
Read 1 review rated 5 using FAB391F in the following applications:
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IGF-I R/IGF1R
Insulin-like Growth Factor I Receptor (IGF-I R) is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I R is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF-I R is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and -II have been shown to be mediated via IGF-I R.
Long Name
Insulin-like Growth Factor I Receptor
Alternate Names
CD221, IGF-1R, IGF-IR, IGF1R, IGFIR
Entrez Gene IDs
3480 (Human)
Gene Symbol
IGF1R
UniProt
Additional IGF-I R/IGF1R Products
Product Documents for Human IGF-I R/IGF1R Fluorescein-conjugated Antibody
Product Specific Notices for Human IGF-I R/IGF1R Fluorescein-conjugated Antibody
For research use only
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