Recombinant Human IFN- alphaI reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN- alphaI (20 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IFN- alphaI Monoclonal Antibody (Catalog # MAB10080). The ND₅₀ is typically 0.6-6 ng/mL.

Applications for Human IFN-alpha I Antibody

Application

Recommended Usage

Neutralization

Measured by its ability to neutralize IFN‑ alphaI inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND₅₀) is typically 0.6-6 ng/mL in the presence of 20 pg/mL Recombinant Human IFN‑ alphaI.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha I

Interferon-alpha (IFN-alpha, IFNA), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. There are at least 18 different IFNA subtypes, and the major subtypes identified are IFNA1, IFNA2, IFNA8, IFNA10, IFNA14 and IFNA21.

Long Name

Interferon alpha I

Alternate Names

IFNA17, IFNalpha I

Entrez Gene IDs

3451 (Human)

Gene Symbol

IFNA17

UniProt

P01571

Additional IFN-alpha I Products

Product Documents for Human IFN-alpha I Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human IFN-alpha I Antibody

For research use only

Citations
Reviews

Human IFN-alpha I Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10080

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all IFN-alpha I Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Endotoxin Level

Scientific Data Images for Human IFN-alpha I Antibody

IFN- alphaI Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN- alphaI Antibody.