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Human HVEM/TNFRSF14 Antibody

Bio-Techne includes R&D Systems | Catalog # AF356

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AF356
AF356-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human HVEM/TNFRSF14 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HVEM/TNFRSF14
Pro37-Val202
Accession # Q92956

Specificity

Detects human HVEM/TNFRSF14 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human HVEM/TNFRSF14 Antibody

Detection of Human HVEM/TNFRSF14 antibody by Western Blot.

Detection of Human HVEM/TNFRSF14 by Western Blot.

Western blot shows lysates of human thymus tissue and human peripheral blood mononuclear cells (PBMCs). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HVEM/TNFRSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for HVEM/TNFRSF14 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HVEM/TNFRSF14 antibody in Human Spleen by Immunohistochemistry (IHC-P).

HVEM/TNFRSF14 in Human Spleen.

HVEM/TNFRSF14 was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human HVEM/TNFRSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF356) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human HVEM/TNFRSF14 by Block/Neutralize

Detection of Human HVEM/TNFRSF14 by Block/Neutralize

Differential inhibition of HVEM/CD160 binding with benchmark tool antibodies. TRF assay measuring the potency of different antibodies to inhibit the binding of recombinant human HVEM-Fc chimera to CD160+ CHO-K1 cells. A) CD160 monoclonal antibodies inhibit binding of HVEM-Fc to both CD160-GPI and CD160-TM isoforms. B) Polyclonal HVEM (left panel) and monoclonal HVEM (right panel) antibodies both enhance binding of HVEM-Fc to CD160-TM isoform. The polyclonal anti-HVEM inhibits HVEM-Fc binding to CD160-GPI (left panel). Antibody concentrations are plotted on the X axis whereas, the calculated percentage of inhibition of binding is plotted on the Y axis. Matched isotype control antibody for each individual antibody candidate was also used in the assay (empty circles and squares). CTL = control, mAb = monoclonal antibody, pAb = polyclonal antibody. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25179432), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human HVEM/TNFRSF14 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen

Western Blot

1 µg/mL
Sample:   Human thymus tissue and human peripheral blood mononuclear cells (PBMCs)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 3 publications using AF356 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HVEM/TNFRSF14

Herpesvirus entry mediator (HVEM), also referred to as TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a type I membrane protein belonging to the TNF/NGF receptor superfamily. In the TNF superfamily nomenclature, HVEM is referred to as TNFRSF14. Human HVEM cDNA encodes a 283 amino acid (aa) residue protein with a probable 36 aa residue signal peptide, a 166 aa residue extracellular domain, a 23 aa residue transmembrane region and a 58 aa residue cytoplasmic region. The extracellular domain of HVEM contains several cysteine-rich repeats characteristic of TNF receptor superfamily members. The short cytoplasmic region lacks a death domain present in some TNF receptor family members, but contains a TRAF (TNF receptor-associated factor) interaction domain. HVEM expression has been detected in peripheral blood T cells, B cells, monocytes and in various tissues enriched in lymphoid cells. The extracellular domain of HVEM has been shown to interact directly with the herpes simplex virus envelope glycoprotein D. Two TNF superfamily ligands, including the secreted TNF‑ beta (lymphotoxin  alpha) and the membrane protein LIGHT (lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), have been shown to be the cellular ligands for HVEM. Besides HVEM, LIGHT can also interact with LT betaR, the receptor for lymphotoxin alpha beta heterotrimer. The role of the HVEM-LIGHT/LT beta receptor-ligand pair in immune function and herpesvirus pathobiology remains to be elucidated.

References

  1. Hsu, H. et al. (1997) J. Biol. Chem. 272:13471.
  2. Mauri, D.N. et al. (1998) Immunity 8:21.
  3. Montgomery, R.I. et al. (1996) Cell 87:427.

Long Name

Herpesvirus Entry Mediator

Alternate Names

ATAR, CD270, LIGHTR, TNFRSF14

Entrez Gene IDs

8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)

Gene Symbol

TNFRSF14

UniProt

Additional HVEM/TNFRSF14 Products

Product Documents for Human HVEM/TNFRSF14 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human HVEM/TNFRSF14 Antibody

For research use only

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