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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Hexosaminidase A/HEXA
Leu23-Thr529
Accession # P06865

Specificity

Detects human Hexosaminidase A/HEXA in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Hexosaminidase A/HEXA

HEXA (beta-hexosaminidase subunit alpha; also hexaminidase subunit alpha) is a 50-56 kDa glycoprotein member of the glycosyl hydrolase #20 family of enzymes. It is found in the lysosomes of a variety of cell types including fibroblasts, lacrimal acinar cells, and neurons. beta-Hexosaminidase is a multimeric enzyme that cleaves aminoacetylhexosamines from multiple glycosylated molecules. It exists in three noncovalent isozyme forms, among which is an A form that is trimeric and composed of one alpha- and two beta-subunits, a B form that is tetrameric and contains two alpha- and two beta-subunits, and a dimeric S form that contains two alpha-subunits. The alpha- and beta-subunits are products of related but distinct genes. The beta-hexosiminidase A form acts on a wide range of substrates, while the B form activity is biased towards sulfated hexoses. Human HEXA is synthesized as a 529 aa (amino acid) prepro-precursor. It contains a 22 aa signal sequence, a 66 aa prosegment, and a 441 aa mature region that contains a catalytic domain (aa 167-488). The prosegment may undergo additional processing through His90, and this segment (≈ 6 kDa) is known to remain linked to the mature region via a disulfide bond. Notably, circulating HEXA is comprised only of subunit proforms whose MW may run some 10 kDa higher in SDS-PAGE. Over aa 23-529, human HEXA shares 85% and 57% aa sequence identity with mouse HEXA and the human HEXA beta-subunit proprecursor, respectively.

Alternate Names

HEXA, TSD

Entrez Gene IDs

3073 (Human)

Gene Symbol

HEXA

UniProt

Additional Hexosaminidase A/HEXA Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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