Human GPRC5B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10253
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 575926
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human GPRC5B
Accession # Q9NZH0
Accession # Q9NZH0
Specificity
Detects human GPRC5B.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human GPRC5B Antibody
Detection of GPRC5B in HEK293 Human Cell Line Transfected with Human GPRC5B and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with (A) GPRC5B or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human GPRC5B Monoclonal Antibody (Catalog # MAB10253) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG1 Isotype Control Antibody staining (Catalog # MAB003, data not shown). View our protocol for Staining Membrane-associated Proteins.Applications for Human GPRC5B Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human GPRC5B and eGFP
Sample: HEK293 Human Cell Line Transfected with Human GPRC5B and eGFP
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPRC5B
G-protein coupled receptor family C group 5-member B (GPRC5B) is a retinoic acid-inducible protein that belongs to the seven-transmembrane domain family of receptors. Human GPRC5B shares approximately 85% aa sequence identity with mouse GPRC5B.
Although GPRC5B is considered an "orphan receptor" and its molecular function is not completely understood, it has been implicated in obesity-associated inflammatory signaling in adipocytes and in diet-induced insulin resistance. Consistent with that, CRISPR-Cas9-mediated knock-down of the GPRC5B gene allowed the identification of signaling pathways linking the GPRC5B receptor expression to beta-cell proliferation and apoptosis.
Long Name
G Protein-coupled Receptor, Family C, Group 5, Member B
Alternate Names
RAIG2
Gene Symbol
GPRC5B
UniProt
Additional GPRC5B Products
Product Documents for Human GPRC5B Antibody
Product Specific Notices for Human GPRC5B Antibody
For research use only
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