Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human Glycine N-methyltransferase/GNMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6526) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Glycine N-methyltransferase/GNMT at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Glycine N-methyltransferase/GNMT Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Capan‑1 human pancreatic adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glycine N-Methyltransferase/GNMT

GNMT (glycine N-methyltransferase) is a cytoplasmic homotetramer of 33 kDa units that converts S-adenosylmethionine to N-methylglycine (sarcosine), also producing S-adenosylhomocysteine. It is abundant in liver, but has also been found in the pancreas and prostate, and is downregulated in hepatocellular carcinoma. GNMT adjusts liver methyl group synthesis for dietary methionine, influencing DNA methylation. The 5-methyltetrahydrofolate form of folic acid binds and inhibits GNMT activity. The 295 amino acid (aa) human GNMT shares 92% aa identity with mouse and rat GNMT.

Alternate Names

Glycine NMethyltransferase, GNMT

Entrez Gene IDs

27232 (Human); 14711 (Mouse); 25134 (Rat)

Gene Symbol

GNMT

UniProt

Q14749 (Human)

Additional Glycine N-Methyltransferase/GNMT Products

Product Documents for Human Glycine N-methyltransferase/GNMT Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Glycine N-methyltransferase/GNMT Antibody

For research use only

Citations
Reviews

Human Glycine N-methyltransferase/GNMT Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6526

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Glycine N-Methyltransferase/GNMT Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Glycine N-methyltransferase/GNMT Antibody

Detection of Human Glycine N-methyltransferase/GNMT by Western Blot.