Human GIF Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8060AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Specificity
Detects human GIF in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human GIF Alexa Fluor™ Plus 488-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Background: GIF
GIF (Gastric Intrinsic Factor; also better known as IF or Intrinsic Factor) is a 45-55 kDa, secreted glycoprotein member of the eukaryotic cobalamin transport family of proteins. It should not be confused with either GIF/growth inhibitory factor/metallothionein 3, or GIF/glycosylation-inhibiting factor/macrophage migration inhibitory factor/phenylpyruvate tautomerase. GIF shows restricted expression, being essentially limited to parietal and select chief cells of the stomach. GIF binds to haptocorrin-associated cobalamin/VitB12 in the duodenum, whereupon it passes into the ileum and is taken up by a 500 kDa epithelial receptor named cubam, a complex of cubilin and amnionless molecules. Mature human GIF is 399 amino acids (aa) in length (aa 19-417) that contains one principal B12 binding site between aa 365-395. There is one isoform variant that shows a deletion of aa 3-27. Full-length human GIF shares 82% aa sequence identity with mouse GIF.
Long Name
Gastric Intrinsic Factor
Alternate Names
IF, IFMH, INF, TCN3
Gene Symbol
CBLIF
UniProt
Additional GIF Products
Product Specific Notices for Human GIF Alexa Fluor™ Plus 488-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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