Human GDNF Alexa Fluor® 488-conjugated Antibody

Name: Human GDNF Alexa Fluor® 488-conjugated Antibody
Brand: R&D Systems
SKU: FAB2121G

R&D Systems, part of Bio-Techne | Catalog # FAB2121G

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Product Datasheet / COA

Key Product Details

Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human GDNF Arg109-Ile211 Accession # P39905
Applications:	Immunocytochemistry/ Immunofluorescence
Label:	Alexa Fluor 488 (Excitation=488 nm, Emission=515-545 nm)
Specificity:	Detects human GDNF in ELISAs

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Technical Data
Citations / Reviews

Applications for Human GDNF Alexa Fluor® 488-conjugated Antibody

	Recommended Concentration	Sample
Immunocytochemistry	Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Protein A or G purified
Formulation	Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: GDNF

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF‑beta superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF‑beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell‑expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Item	Value
Long Name	Glial Cell line-derived Growth Factor
Entrez Gene IDs	2668 (Human); 25453 (Rat)
Alternate Names	ATF, ATF1, ATF2, Astrocyte-derived trophic factor, GDNF, HFB1-GDNF, HGDNF, HSCR3, glial cell derived neurotrophic factor, glial cell line derived neurotrophic factor, glial cell line-derived neurotrophic factor

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Product Documents for Human GDNF Alexa Fluor® 488-conjugated Antibody

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