Sign In / Register
Human GATA‑3 PE‑conjugated Antibody
Catalog # IC26051P | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 291106
Product Summary for Human GATA‑3 PE‑conjugated Antibody
Immunogen
E. coli-derived recombinant human GATA-3
Pro135-Ser258
Accession # P23771
Pro135-Ser258
Accession # P23771
Specificity
Detects human GATA-3 in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant human GATA-1, -2, -4, -5, or ‑6.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human GATA‑3 PE‑conjugated Antibody
Detection of GATA‑3 in MCF‑7 Human Cell Line by Flow Cytometry.
MCF-7 human breast cancer cell line was stained with Mouse Anti-Human GATA-3 PE-conjugated Monoclonal Antibody (Catalog # IC26051P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications for Human GATA‑3 PE‑conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: MCF‑7 human breast cancer cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Sample: MCF‑7 human breast cancer cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using IC26051P in the following applications:
Published Applications
Read 2 publications using IC26051P in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: GATA-3
GATA-3 belongs to the GATA family of transcription factors, which bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. It is widely expressed in mesodermal- and endodermal-derived tissues. GATA-3 has been shown to be an essential regulator for immune cell function, sympathetic neuron development, and the maintenance of the differentiated state in epithelial cells.
Product Documents for Human GATA‑3 PE‑conjugated Antibody
Product Specific Notices for Human GATA‑3 PE‑conjugated Antibody
For research use only
Citations for Human GATA‑3 PE‑conjugated Antibody (2)
Showing
1
-
2 of
2 publications
Showing All
Filter By:
Search Title:
Citations are publications that use Bio-Techne products. Selected citations for Human GATA‑3 PE‑conjugated Antibody include:
-
Species: Canine
Sample Types: Whole Cells
Applications: Flow CytometrySF Costa et al. (2020-01-21), Combined in vitro IL-12 and IL-15 stimulation promotes cellular immune response in dogs with visceral leishmaniasis PLoS Negl Trop Dis, 2020-014(1):e0008021.
PMID: 31961868 -
Species: Canine
Sample Types: Whole Cells
Applications: Flow CytometryLM Melo et al. (2019-12-11), Induction of miR 21 impairs the anti-Leishmania response through inhibition of IL-12 in canine splenic leukocytes PLoS ONE, 2019-114(12):e0226192.
PMID: 31825987
There are no citations that match your criteria.
Customer Reviews for Human GATA‑3 PE‑conjugated Antibody (1)
Loading...
