Western blot shows lysates of human brain (cerebellum) tissue, human brain (hypothalamus) tissue, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Recombinant Mouse Anti-Human gamma-Synuclein Monoclonal Antibody (Catalog # MAB5745R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for gamma-Synuclein at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

gamma-Synuclein in MBA‑MB‑468 Human Cell Line.

gamma-Synuclein was detected in immersion fixed MBA-MB-468 human breast cancer cell line using Recombinant Mouse Anti-Human gamma-Synuclein Monoclonal Antibody (Catalog # MAB5745R) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

gamma-Synuclein in Human Brain.

gamma-Synuclein was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Recombinant Mouse Anti-Human gamma-Synuclein Monoclonal Antibody (Catalog # MAB5745R) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human gamma-Synuclein Antibody

Application

Recommended Usage

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed MBA‑MB‑468 human breast cancer cell line

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Western Blot

0.5 µg/mL
Sample: Human brain (cerebellum) tissue, human brain (hypothalamus) tissue, and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: gamma-Synuclein

gamma-Synuclein (SNCG), also called persyn or synoretin, is a 17 kDa, 127 amino acid (aa) cytoplasmic phosphoprotein. It is a member of a family of small, highly conserved synuclein proteins localized predominantly in presynaptic nerve terminals in the brain. SNCG and other synucleins are implicated in neurodegenerative diseases including Parkinson's disease. SNCG expression is elevated in many cancers and is implicated in tumor progression, invasion and proliferation. Within the region used as an immunogen, human SNCG shares 82% and 78% aa identity with mouse and rat SNCG, respectively.

Alternate Names

BCSG1, PRSN, SNCG, Synoretin, Synuclein-gamma

Entrez Gene IDs

6623 (Human)

Gene Symbol

SNCG

UniProt

Q6FHG5

Additional gamma-Synuclein Products

Product Documents for Human gamma-Synuclein Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human gamma-Synuclein Antibody

For research use only

Citations
Reviews

Human gamma-Synuclein Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5745R

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all gamma-Synuclein Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human gamma-Synuclein Antibody

Detection of Human gamma-Synuclein by Western Blot.