Human Galectin-3 Antibody

Name: Human Galectin-3 Antibody
Brand: R&D Systems
SKU: MAB1154

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB1154

Citations (3)

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Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human Galectin‑3 Ala2-Ile250 Accession # P17931
Applications:	Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2B Clone # 194804
Specificity:	Detects human Galectin-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows 100% cross-reactivity with recombinant mouse Galectin-3 and no cross-reactivity with rhGalectin-1, -2 -4, -7, or -8.

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Applications for Human Galectin-3 Antibody

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Galectin-3 Antibody

	Western Blot Detection of Human Galectin‑3 by Western Blot. Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Galectin-3 Monoclonal Antibody (Catalog # MAB1154) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Galectin-3

Galectin-3, also known as Mac-2, L29, CBP35 and epsilon BP, is a member of a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified to date. Galectin-3 is expressed in tumor cells, macrophages, activated T cells, osteoclasts, epithelial cells and fibroblasts.

Item	Value
Entrez Gene IDs	3958 (Human); 16854 (Mouse)
Alternate Names	AGE-R3, CBP 35,35 kDa lectin, CBP35, Carbohydrate-binding protein 35, GAL3, GALBPCBP35, GALIG, Gal-3, Galactose-specific lectin 3, Galactoside-binding protein, Galectin-3, Galectin3, IgE-binding protein, L-31, L29, L31, LGALS2, LGALS3, Laminin-binding protein, Lectin L-29, MAC2GAL3, Mac-2, Mac-2 antigen, galactin-3, lectin, galactoside-binding, soluble, 3

Citations for Human Galectin-3 Antibody (3)

Showing 1 - 3 of 3 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human Galectin-3 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Suzuki O et al. (2012), Galectin-1-mediated cell death is increased by CD30-induced signaling in anaplastic large cell lymphoma cells but not in Hodgkin lymphoma cells. Lab. Invest., 92(2):191-9.
PMID: 21986812
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Spivey KA et al. (2012), A role for collagen XXIII in cancer cell adhesion, anchorage-independence and metastasis. Oncogene, 31(18):2362-72.
PMID: 21963851
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

Papaspyridonos M et al. (2007), Galectin-3 is an amplifier of inflammation in atherosclerotic plaque progression through macrophage activation and monocyte chemoattraction. Arterioscler. Thromb. Vasc. Biol., 28(3):433-40.
PMID: 18096829

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Human Galectin-3 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human Galectin-3 Antibody

Scientific Data Examples for Human Galectin-3 Antibody

Western Blot

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Galectin-3

Citations for Human Galectin-3 Antibody (3)

Product Documents for Human Galectin-3 Antibody

Product Datasheets

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