25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Fucosyltransferase 3/FUT3 (Catalog # 4950-GT), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Fucosyltransferase 3/FUT3 (Catalog # 4950-GT)
Recombinant Human Fucosyltransferase 5/FUT5 (Catalog # 4949-GT)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fucosyltransferase 3/5 (FUT3/5)

FUT5 ( alpha-1, 3-fucosyltransferase 5; also Fuc-TV) is a 43 kDa member of the glycosyltransferase 10 family. It is responsible for the terminal step in the synthesis of Le^x, sialyl‑Le^x, and Le^a antigens. This is accomplished by the transfer of fucose to preexisting LacNAc or sialyl-LacNAc structures. Human FUT5 is 374 amino acids (aa) in length. It is a type II transmembrane Golgi glycoprotein with a C-terminal lumenal domain (aa’s 35-374). The catalytic domain spans aa’s 77-374, while the acceptor-binding motif includes aa’s 120-126. FUT5 is the product of a single exon gene. Thus, there are no splice variants. FUT5 only occurs in primates; there is no rodent ortholog.

Alternate Names

CD174, FT3B, FucT-III, LE, Les

Entrez Gene IDs

2525 (Human)

Gene Symbol

FUT3

UniProt

Q11128

Additional Fucosyltransferase 3/5 (FUT3/5) Products

Product Documents

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices

For research use only

Citations
Reviews

Human Fucosyltransferase 3/5 (FUT3/5) Lumenal Domain Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4068

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Fucosyltransferase 3/5 (FUT3/5) Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype