Human FoxP3 Biotinylated Antibody

Name: Human FoxP3 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF3240
Price: 483 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF3240

Citations (1)

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Species Reactivity:	Human
Immunogen:	E. coli-derived recombinant human FoxP3 isoform 1 Gln105-Lys200 Accession # Q9BZS1
Applications:	Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects human FoxP3 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human FoxD3 is observed.

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Applications for Human FoxP3 Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human FoxP3

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: FoxP3

Human FoxP3 is a 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three isoforms for FoxP3 have been reported. All three isoforms share the sequence used as the immunogen. FoxP3 directly associates with NFAT and NFkB, suppressing their activity in CD4⁺ T cells. In human, FoxP3 is found in CD4⁺, CD8⁺ and CD4⁺CD25⁺ T cells. Over the region used for immunization of the amino acid sequence, mouse FoxP3 is 83% to 88% identical to rat, human, canine, and bovine FoxP3.

Item	Value
Long Name	Forkhead Box P3
Entrez Gene IDs	50943 (Human); 20371 (Mouse)
Alternate Names	AIID, AIIDMGC141961, DIETER, FOXP3delta7, Forkhead Box Protein P3, FoxP3, IPEX, Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked, JM2, MGC141961, MGC141963, PIDX, PIDXMGC141963, SCURFIN, XPID, XPIDpolyendocrinopathy, enteropathy, X-linked, forkhead box P3, immune dysregulation, polyendocrinopathy, enteropathy, X-linked

Citations for Human FoxP3 Biotinylated Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human FoxP3 Biotinylated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Barboza L et al. (2007-01-01), Antigen-induced regulatory T cells in HBV chronically infected patients. Virology, 368(1):41-9.
PMID: 17643462

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Human FoxP3 Biotinylated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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