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Human FGF-19 Antibody

Bio-Techne includes R&D Systems | Catalog # AF969

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AF969-SP
AF969

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Blockade of Receptor-ligand Interaction, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human FGF-19 Antibody

Immunogen

E. coli-derived recombinant human FGF-19
Phe27-Lys216
Accession # O95750

Specificity

Detects human FGF-19 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) FGF-7, rhFGF-21 and recombinant mouse FGF-15 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human FGF-19 Antibody

Detection of Human FGF-19 antibody by Western Blot.

Detection of Human FGF‑19 by Western Blot.

Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, conditioned media from COLO 205 cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human FGF-19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF969) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FGF-19 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human FGF-19 antibody by Simple WesternTM.

Detection of Human FGF‑19 by Simple WesternTM.

Simple Western lane view shows conditioned media from COLO 205 human colorectal adenocarcinoma cell line and HT-29 human colon adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for FGF-19 at approximately 26 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FGF-19 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF969) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Applications for Human FGF-19 Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 2-8 µg/mL of this antibody will block 50% of the binding of 250 ng/mL of Recombinant Human FGF-19 (Catalog # 969-FG) to immobilized Recombinant Human FGF R4 Fc Chimera (Catalog # 685-FR) coated at 2 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.

Simple Western

10 µg/mL
Sample: Conditioned media from COLO 205 human colorectal adenocarcinoma cell line and HT‑29 human colon adenocarcinoma cell line

Western Blot

0.5 µg/mL
Sample: Conditioned media from COLO 205 cell line and HT‑29 human colon adenocarcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 7 publications using AF969 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-19

Fibroblast growth factor 19 (FGF-19) belongs to the large FGF family which has at least 23 members (1, 2). All FGF family members are heparin-binding growth factors with a core 120 amino acid (aa) FGF domain that allows for a common tertiary structure. FGFs are expressed during embryonic development and in restricted adult tissues. They act on cells of mesodermal and neuroectodermal origin to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects and tissue repair (3, 4). Signaling receptors for FGFs are type I transmembrane receptor tyrosine kinases belonging to the Ig superfamily. Four distinct but related classes of FGF receptors, FGF R1, 2, 3, and 4, exist. Through alternative splicing, multiple isoforms for FGF R1, 2 and 3, with distinct ligand recognition profiles, are also generated (4).

Human FGF-19 cDNA predicts a 251 aa precursor protein with a 22 aa signal peptide and a 229 aa secreted mature protein with no potential N-linked glycosylation sites (1, 2). Among FGF family members, human FGF-19 is most closely related to chicken FGF-19 and murine FGF-15, sharing approximately 61% and 51% aa sequence identity, respectively (1, 2, 5). Neither the human orthologue of mouse FGF-15, nor the mouse counterpart of human FGF-19 has been identified. With the exception of adult gall bladder epithelium, FGF-19 expression is restricted to fetal tissues (1, 2). Unlike most FGFs which bind to and activate more than one FGF receptor, FGF-19 is a specific ligand for FGF R4 (2). Similarly, another FGF family member, FGF-7 (KGF), only activates KGF R, the IIIb isoform of FGF R2 (4). During chick embryogenesis, FGF-19 has been shown to act synergistically with Wnt-8c to initiate inner ear development (5).

References

  1. Nishimura, T. et al. (1999) Biocheim. Biophys. Acta 1444:148.
  2. Xie, M. et al. (1999) Cytokine 11:729.
  3. Goldfarb, M. (1996) Cytokine & Growth Factor Reviews 7:311.
  4. Green, P. et al. (1996) BioEssays 18:639.
  5. Ladher, R.K. et al. (2000) Science 290:1965.

Long Name

Fibroblast Growth Factor 19

Alternate Names

FGF19

Entrez Gene IDs

9965 (Human)

Gene Symbol

FGF19

UniProt

Additional FGF-19 Products

Product Documents for Human FGF-19 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FGF-19 Antibody

For research use only

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