Human Fas Ligand/TNFSF6 Antibody
Catalog # AF126 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
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Product Summary for Human Fas Ligand/TNFSF6 Antibody
Immunogen
Pro134-Leu281
Accession # Q53ZZ1
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Fas Ligand/TNFSF6 Antibody
Detection of Fas Ligand/TNFSF6 in HEK293 Human Cell Line Transfected with Human Fas Ligand/TNFSF6 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with (A) Fas Ligand/TNFSF6 or (B) irrelevant protein, and eGFP were stained with Goat Anti-Human Fas Ligand/TNFSF6 Affinity Purified Polyclonal Antibody (Catalog # AF126) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). Quadrant markers were set based Goat IgG Control Antibody staining (Catalog # AB-108-C, data not shown). View our protocol for Staining Membrane-associated Proteins.Fas Ligand/TNFSF6 in Human Melanoma.
Fas Ligand/TNFSF6 was detected in immersion fixed paraffin-embedded sections of human melanoma tissue using Goat Anti-Human Fas Ligand/TNFSF6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF126) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.Fas Ligand/TNFSF6 in Human Thymus.
Fas Ligand/TNFSF6 was detected in immersion fixed paraffin-embedded sections of human thymus using Goat Anti-Human Fas Ligand/TNFSF6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF126) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013)). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Fas Ligand/TNFSF6 Antibody
Flow Cytometry
Sample: HEK293 Human Cell Line Transfected with Human Fas Ligand/TNFSF6 and eGFP
Immunohistochemistry
Sample: Immersion fixed paraffin-embedded sections of human melanoma tissue and human thymus
Western Blot
Sample: Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL)
Neutralization
Formulation, Preparation and Storage
Purification
Endotoxin Level
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fas Ligand/TNFSF6
References
- Lettau, M. et al. (2008) Curr. Med. Chem. 15:1684.
- Takahashi, T. et al. (1994) Int. Immunol. 6:1567.
- Seino, K-I. et al. (1998) J. Immunol. 161:4484.
- Suda, T. et al. (1993) Cell 75:1169.
- Pitti, R.M. et al. (1998) Nature 396:699.
- Schneider, P. et al. (1998) J. Exp. Med. 187:1205.
- Tanaka, M. et al. (1998) Nature Med. 4:31.
- Chen, J-J. et al. (1998) Science 282:1714.
- Suzuki, I. and P.J. Fink (2000) Proc. Natl. Acad. Sci. USA 97:1707.
- Ferguson, T.A. and T.S. Griffith (2006) Immunol. Rev. 213:228.
- Ryan, A.E. et al. (2005) Cancer Res. 65:9817.
- Takahashi, T. et al. (1994) Cell 76:969.
- Lynch, D.H. et al. (1994) Immunity 1:131.
Alternate Names
Gene Symbol
UniProt
Product Documents for Human Fas Ligand/TNFSF6 Antibody
Product Specific Notices for Human Fas Ligand/TNFSF6 Antibody
For research use only
Citations for Human Fas Ligand/TNFSF6 Antibody (5)
Citations are publications that use Bio-Techne products. Selected citations for Human Fas Ligand/TNFSF6 Antibody include:
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Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: Western Blot,Flow Cytometry,NeutralizationWR English et al. (2018-04-04), Tissue Inhibitor of Metalloproteinase-3 (TIMP-3) induces FAS dependent apoptosis in human vascular smooth muscle cells PLoS ONE, 2018-013(4):e0195116.
PMID: 29617412 -
Species: Human
Sample Types: Whole Cells
Applications: BioassaySiewiera J et al. (2013-04-04), Human cytomegalovirus infection elicits new decidual natural killer cell effector functions. PLoS Pathog, 2013-09(4):e1003257.
PMID: 23592985 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationBeynon V et al. (2012-06-29), Activated Human CD4+CD45RO+ Memory T-Cells Indirectly Inhibit NLRP3 Inflammasome Activation through Downregulation of P2X7R Signalling. PLoS ONE, 2012-07(6):e39576.
PMID: 22768094 -
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: Western Blot,ICCKim YC et al. (2012-05-18), Thymosin beta10 expression driven by the human TERT promoter induces ovarian cancer-specific apoptosis through ROS production. PLoS ONE, 2012-07(5):e35399.
PMID: 22623951 -
Species: Human
Sample Types: Serum
Applications: NeutralizationHunger RE et al. (2005-08-19), Rapid resolution of toxic epidermal necrolysis with anti-TNF-alpha treatment. J. Allergy Clin. Immunol., 2005-0116(4):923-4.
PMID: 16210071
There are no citations that match your criteria.
Customer Reviews for Human Fas Ligand/TNFSF6 Antibody (1)
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