Detects human FAM3C in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) FAM3A, rhFAM3B, recombinant mouse (rm) FAM3C, or rmFAM3D is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human FAM3C Antibody

FAM3C in A549 Human Cell Line.

FAM3C was detected in immersion fixed A549 human lung carcinoma cells treated with 10ng/mL of Recombinant Human TGF-beta 1 (Catalog # 240-B) for 48 hours using Mouse Anti-Human FAM3C Monoclonal Antibody (Catalog # MAB3084) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human FAM3C Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cells treated with 10ng/mL of Recombinant Human TGF-beta 1 (Catalog # 240-B)

Western Blot

1 µg/mL
Sample: Recombinant Human FAM3C

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FAM3C

FAM3C (Family with sequence similarity 3, member C), also known as D6WSU176e, is a secreted protein belonging to a cytokine-like family. Members of this family share a four helix bundle secondary structure similar to that found in many cytokines. FAM3C is ubiquitously expressed in multiple tissues. The human FAM3C gene has been identified to be a candidate gene for autosomal recessive non-syndromic hearing loss locus 17. The amino acid sequence of mature mouse FAM3C is 97% and 94% identical to that of rat and human FAM3C, respectively.

Long Name

Family With Sequence Similarity 3, Member C

Alternate Names

D6WSU176e, ILEI

Entrez Gene IDs

10447 (Human); 27999 (Mouse); 312159 (Rat)

Gene Symbol

FAM3C

UniProt

Q92520

Additional FAM3C Products

Product Documents for Human FAM3C Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human FAM3C Antibody

For research use only

Citations
Reviews

Human FAM3C Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3084

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all FAM3C Antibodies »

Product Specifications

Immunogen

Specificity