Human ESAM Antibody

Name: Human ESAM Antibody
Brand: R&D Systems
SKU: AF2688

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF2688

Citations (2)

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human ESAM Gln30-Ala247 Accession # Q96AP7
Applications:	CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human ESAM in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant mouse ESAM is observed.

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Applications for Human ESAM Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Flow Cytometry	2.5 µg/10⁶ cells	See below
Immunohistochemistry	5-15 µg/mL	Immersion fixed paraffin-embedded sections of human kidney and liver
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human ESAM Antibody

	Western Blot Detection of Human ESAM by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ESAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2688) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ESAM at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Flow Cytometry Detection of ESAM in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human ESAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2688, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: ESAM

Endothelial cell-selective adhesion molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules (1, 2). Human ESAM is synthesized as a 390 amino acid (aa) protein composed of a 29 aa signal peptide, a 216 aa extracellular region, a putative 26 aa transmembrane segment, and a 119 aa cytoplasmic domain. The extracellular region contains one V-type and one C2-type Ig domain and is involved in homophilic adhesion (1). In the cytoplasmic domain, there is a docking site for the multifunctional adaptor protein MAGI-1 (3). The extracellular region of human ESAM shows 90%, 74%, 69% and 67% aa identity with monkey, canine, mouse and rat extracellular ESAM, respectively. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes, and can be found associated with cell-to-cell junctions. Whether ESAM is restricted to a particular junctional type is not clear (1, 2). ESAM deficient mice have no defect in vascularization but do have reduced angiogenic potential. This may be due to a decreased migratory response to FGF-2 (4).

Item	Value
References	Hirata, K-I, et al. (2001) J. Biol. Chem. 276:16223. Nasdala, I. et al. (2002) J. Biol. Chem. 277:16294. Wegmann, F. et al. (2004) Exp. Cell Res. 300:121. Ishida, T. et. al. (2003) J. Biol. Chem. 278:34598.
Long Name	Endothelial Cell Adhesion Molecule
Entrez Gene IDs	90952 (Human); 69524 (Mouse)
Alternate Names	2310008D05Rik, ESAM, HUEL (C4orf1)-interacting protein, LP4791 protein, W117m, endothelial cell adhesion molecule, endothelial cell-selective adhesion molecule

Citations for Human ESAM Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human ESAM Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC

NR Hansmeier et al. (2022), 3D Visualization of Human Blood Vascular Networks Using Single-Domain Antibodies Directed against Endothelial Cell-Selective Adhesion Molecule (ESAM) International Journal of Molecular Sciences, 23(8).
PMID: 35457187
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Roesli C et al. (2008), Identification of the surface-accessible, lineage-specific vascular proteome by two-dimensional peptide mapping. FASEB J., 22(6):1933-44.
PMID: 18180333

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Human ESAM Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Scientific Data Examples for Human ESAM Antibody

Western Blot

Flow Cytometry