Human Erythropoietin/EPO Antibody

Name: Human Erythropoietin/EPO Antibody
Brand: R&D Systems
SKU: MAB287

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB287

Citations (5)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Chinese hamster ovary cell line CHO-derived recombinant human Erythropoietin/EPO Accession # P01588
Applications:	Block/Neutralize
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 9C21D11
Specificity:	Detects human Erythropoietin/EPO.

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Applications for Human Erythropoietin/EPO Antibody

Item	Value
Neutralization	Measured by its ability to neutralize Erythropoietin/EPO-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND₅₀) is typically 0.5-1.5 µg/mL in the presence of 0.3 units/mL Recombinant Human Erythropoietin/EPO (Tissue Culture Grade).

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Erythropoietin/EPO Antibody

	Neutralization Cell Proliferation Induced by Erythropoietin/EPO and Neutralization by Human Erythropoietin/EPO Antibody. Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (Catalog # 287-TC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Erythropoietin/EPO (Tissue Culture Grade) (0.3 units/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB287). The ND₅₀ is typically 0.5-1.5 µg/mL.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Erythropoietin/EPO

Erythropoietin (EPO) a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells.

Item	Value
Entrez Gene IDs	2056 (Human); 13856 (Mouse); 24335 (Rat)
Alternate Names	ECYT5, EP, EPO, Erythropoietin, MGC138142, MVCD2, epoetin

Citations for Human Erythropoietin/EPO Antibody (5)

Showing 1 - 5 of 5 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human Erythropoietin/EPO Antibody include:

Species: Transgenic Hamster
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture)

A Costello et al. (2019), Continuous Translation of Circularized mRNA Improves Recombinant Protein Titer Metab. Eng., 0(0).
PMID: 30615942
Species: Rat
Sample Types: In Vivo
Applications: Neutralization

Kondo A et al. (2009), Erythropoietin exerts anti-epileptic effects with the suppression of aberrant new cell formation in the dentate gyrus and upregulation of neuropeptide Y in seizure model of rats. Brain Res., 1296(0):127-36.
PMID: 19695235
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

MacRedmond R et al. (2009), Erythropoietin inhibits respiratory epithelial cell apoptosis in a model of acute lung injury. Eur. Respir. J., 33(6):1403-14.
PMID: 19164355
Kirkeby A et al. (0), Functional and immunochemical characterisation of different antibodies against the erythropoietin receptor. J Neurosci Method164(1):50-8.
PMID: 17524492
Species: Human
Sample Types: Recombinant Protein
Applications: ELISA Development (Capture)

Fliedl L et al. (0), Novel human renal proximal tubular cell line for the production of complex proteins. J Biotechno176(0):29-39.
PMID: 24553072

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Showing 1 - 5 of 5 citations Showing All

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