Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB9589R
Recombinant Monoclonal Antibody For Research Use Only
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu5
Product Specifications
Immunogen
Human ErbB2/Her2
Specificity
Detects human ErbB2/Her2 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Trastuzumab. This product is for research use only.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Scientific Data Images
Detection of ErbB2/Her2 in MCF-7 human cell line by Flow Cytometry
MCF-7 human cell line was stained with Human Anti-Human ErbB2/Her2 (Research Grade Trastuzumab Biosimilar) Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB9589R, filled histogram) or irrelevant antibody (open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of ErbB2/Her2 by Immunohistochemistry
Antibody permeability and accumulation dynamics within an in vitro metastatic blood-tumor barrier model. a Representative images of antibody permeability with and without cancer cells. Images are at 30 min after perfusion with non-specific or anti-HER2 IgG in BBB and BTB microvessels. iBMECs (red), non-specific IgG (blue), anti-HER2 IgG (magenta), JIMT-1-BR (green). b Quantification of focal leaks between BBB and BTB microvessels over time (n = 4–5 independent microvessels per condition). c Representative images of antibody accumulation within the BBB and BTB at day 4. Images are normalized to day 2 fluorescence. d Quantification of antibody accumulation across BBB endothelium, BTB endothelium, and cancer spheroids (n = 4 independent microvessels per condition). Data are presented as mean ± SD. *p < 0.05. See also Additional file 1: Fig. S2 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37924145), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of ErbB2/Her2 by Immunocytochemistry/ Immunofluorescence
Antibody permeability and accumulation dynamics within an in vitro metastatic blood-tumor barrier model. a Representative images of antibody permeability with and without cancer cells. Images are at 30 min after perfusion with non-specific or anti-HER2 IgG in BBB and BTB microvessels. iBMECs (red), non-specific IgG (blue), anti-HER2 IgG (magenta), JIMT-1-BR (green). b Quantification of focal leaks between BBB and BTB microvessels over time (n = 4–5 independent microvessels per condition). c Representative images of antibody accumulation within the BBB and BTB at day 4. Images are normalized to day 2 fluorescence. d Quantification of antibody accumulation across BBB endothelium, BTB endothelium, and cancer spheroids (n = 4 independent microvessels per condition). Data are presented as mean ± SD. *p < 0.05. See also Additional file 1: Fig. S2 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37924145), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: MCF-7 Human cell line
Sample: MCF-7 Human cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ErbB2/Her2
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Alternate Names
CD340, HER2, Neu Oncogene, NGL, TKR1
Gene Symbol
ERBB2
Additional ErbB2/Her2 Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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