Human EpCAM/TROP-1 Antibody

Name: Human EpCAM/TROP-1 Antibody
Brand: R&D Systems
SKU: AF960
Price: 152 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF960

Citations (11)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human EpCAM/TROP-1, extracellular domain Gln24-Lys265 Accession # CAA32870
Applications:	CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human EpCAM/TROP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human TROP-2 and approximately 26% cross-reactivity with recombinant mouse EpCAM/TROP-1 is observed.

View Full Technical Details for Human EpCAM/TROP-1 Antibody

Applications for Human EpCAM/TROP-1 Antibody

	Recommended Concentration	Sample
Western Blot	0.2 µg/mL	See below
Simple Western	10 µg/mL	A549 human lung carcinoma cell line exosomes, HT‑29 human colon adenocarcinoma cell line exosomes, and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL)
Flow Cytometry	0.25 µg/10⁶ cells	See below
Immunohistochemistry	0.3-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human EpCAM/TROP-1 Antibody

	Western Blot Detection of Human EpCAM/TROP‑1 by Western Blot. Western blot shows lysates of HCT-116 human colorectal carcinoma cell line, MCF-7 human breast cancer cell line, and BG01V human embryonic stem cells. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for EpCAM/TROP-1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Flow Cytometry Detection of EpCAM/TROP‑1 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960, filled histogram) or isotype control antibody (AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
	Immunocytochemistry EpCAM/TROP‑1 in BG01V Human Embryonic Stem Cells. EpCAM/TROP-1 was detected in immersion fixed BG01V human embryonic stem cells using Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunohistochemistry EpCAM/TROP‑1 in Human Colon. EpCAM/TROP-1 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
	Immunohistochemistry EpCAM/TROP‑1 in Human Colon. EpCAM/TROP-1 was detected in immersion fixed paraffin-embedded sections of human colon using Goat Anti-Human EpCAM/TROP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
	Simple Western Detection of Human EpCAM/TROP‑1 by Simple Western^TM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line exosomes, HT‑29 human colon adenocarcinoma cell line exosomes, and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for EpCAM/TROP‑1 at approximately 52 kDa (as indicated) using 10 µg/mL of Goat Anti-Human EpCAM/TROP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
	Immunocytochemistry Detection of EpCAM/TROP‑1 in HT-29 Human Colon Adenocarcinoma Cell Line (Positive) and Jurkat Human Acute T Cell Leukemia Cell Line (Negative) Cells. EpCAM/TROP‑1 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line (positive) and Jurkat human acute T cell leukemia cell line (negative) cells using Goat Anti-Human EpCAM/TROP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF960) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP-1, is a 40 kDa transmembrane glycoprotein composed of a 242 amino acid (aa) extracellular domain with two epidermal-growth-factor-like (EGF-like) repeats within the cysteine-rich N-terminal region, a 23 aa transmembrane domain, and a 26 aa cytoplasmic domain. Human and mouse EpCAM share 82% aa sequence identity. In human, EpCAM also shares 49% aa sequence homology with TROP-2/EGP-1. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (1). EpCAM expression has been found to increase in actively proliferating epithelia tissues and during adult liver regeneration (1, 2). EpCAM expression is also found to increase in human malignant neoplasias, with most carcinoma expressing EpCAM including those of arising from squamous epithelia (1). EpCAM has been shown function as a homophilic Ca²⁺ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF-like repeats, with the first EGF-like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (4). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha -actinin (5).

Item	Value
References	Balzar, M. et al. (1999) J. Mol. Med. 77:699. Boer, C.J. et al. (1999) J. Pathol. 188:201. Litvinow, S.V. et al. (1994) J. Cell Biol. 125:437. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570. Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.
Long Name	Epithelial Cell Adhesion Molecule
Entrez Gene IDs	4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)
Alternate Names	17-1A, 323/A3, ACSTD1, CD326, CD326 antigen, Cell surface glycoprotein Trop-1, DIAR5, EGP, EGP-2, EGP314, EGP40, ESA, EpCAM, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, GA733-2, GA733-2EGP34, HNPCC8, KS 1/4 antigen, KS1/4, KSAHEA125, M1S2, M4S1, M4S1Ly74, MIC18MH99, MOC31, Major gastrointestinal tumor-associated protein GA733-2, TACST-1, TACSTD1, TROP1, TROP1CD326, Tumor-associated calcium signal transducer 1CO-17A, antigen identified by monoclonal AUA1, chromosome 4, surface marker (35kD glycoprotein), epithelial cell adhesion molecule, gp40, hEGP314

Citations for Human EpCAM/TROP-1 Antibody (11)

Showing 1 - 10 of 11 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Human EpCAM/TROP-1 Antibody include:

Species: Human
Sample Types: Urine
Applications: ELISA Capture

KM Chan et al. (2021-01-01), Improving hexaminolevulinate enabled cancer cell detection in liquid biopsy immunosensors Scientific Reports, 11(1):7283.
PMID: 33790357
Species: Mouse
Sample Types: Whole Cells
Applications: IHC

H Yang et al. (2021-01-01), Targeted Inhibition of LPL/FABP4/CPT1 fatty acid metabolic axis can effectively prevent the progression of nonalcoholic steatohepatitis to liver cancer International journal of biological sciences, 17(15):4207-4222.
PMID: 34803493
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

J Zhang et al. (2021-01-01), Immunomagnetic sequential ultrafiltration (iSUF) platform for enrichment and purification of extracellular vesicles from biofluids Scientific Reports, 11(1):8034.
PMID: 33850163
Species: Human
Sample Types: Whole Cells
Applications: ICC

S Yoshida et al. (2020-01-01), Generation of intestinal organoids derived from human pluripotent stem cells for drug testing Sci Rep, 10(1):5989.
PMID: 32249832
Species: Human
Sample Types: Plasma
Applications: ELISA Capture

N Sun et al. (2020-01-01), Purification of HCC-specific extracellular vesicles on nanosubstrates for early HCC detection by digital scoring Nat Commun, 11(1):4489.
PMID: 32895384
Species: Human
Sample Types: Whole Cells
Applications: CyTof

LL Lin et al. (2020-01-01), PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer Cell Rep, 33(2):108253.
PMID: 33053339
Species: Human
Sample Types: Whole Cells
Applications: ICC

D Garnier et al. (2018-01-01), Expansion of human primary hepatocytes in vitro through their amplification as liver progenitors in a 3D organoid system Sci Rep, 8(1):8222.
PMID: 29844473
Species: Human
Sample Types: Whole Cells
Applications: Adhesion Assay

(2016-01-01), EpCAM-Regulated Transcription Exerts Influences on Nanomechanical Properties of Endometrial Cancer Cells that Promote Epithelial-to-Mesenchymal Transition Cancer Res, 0(0).
PMID: 27569206
Species: Human
Sample Types: Whole Cells
Applications: Cell Selection

(2016-01-01), Fast and selective cell isolation from blood sample by microfiber fabric system with vacuum aspiration Sci Technol Adv Mater, 17(1):807-815.
PMID: 27933120
Species: Human
Sample Types: Whole Blood
Applications: CTC

(2016-01-01), HER2 and EGFR overexpression support metastatic progression of prostate cancer to bone Cancer Res., 0(0).
PMID: 27793843
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Calon A et al. (2015-01-01), Stromal gene expression defines poor-prognosis subtypes in colorectal cancer. Nat Genet, 47(4):320-9.
PMID: 25706628

There are no citations that match your criteria.

Showing 1 - 10 of 11 citations Showing All

Product Documents for Human EpCAM/TROP-1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Human EpCAM/TROP-1 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased

Applications for Human EpCAM/TROP-1 Antibody

Scientific Data Examples for Human EpCAM/TROP-1 Antibody

Western Blot

Flow Cytometry

Immunocytochemistry

Immunohistochemistry

Immunohistochemistry

Simple Western