Human EGFR F(ab')2 (Research Grade Cetuximab Biosimilar) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9577-FAB2
Recombinant Monoclonal Antibody For Research Use Only
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu1F2
Product Specifications
Immunogen
Human EGFR/ErbB1
Specificity
Detects
human EGFR based on Cetuximab therapeutic antibody. This
non-therapeutic antibody uses the same variable region sequence as the
therapeutic antibody Cetuximab. This product is for research use only.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Scientific Data Images for Human EGFR F(ab')2 (Research Grade Cetuximab Biosimilar) Antibody
Detection of EGFR in A431 cells by Flow Cytometry.
A431 cells were stained with Human Anti-Human EGFR F(ab')2 (Research Grade Cetuximab Biosimilar) Monoclonal Antibody (Catalog # MAB9577-fab2, filled histogram) or isotype control antibody (Catalog # 1-001-A, open histogram), followed by Goat Anti-Human IgG Allophycocyanin (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.Applications for Human EGFR F(ab')2 (Research Grade Cetuximab Biosimilar) Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: A431 human epithelial carcinoma cell line
Sample: A431 human epithelial carcinoma cell line
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Sample: Immersion fixed A431 human epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EGFR
Long Name
Epidermal Growth Factor Receptor
Alternate Names
EGF R, ErbB, ErbB1, HER-1
Gene Symbol
EGFR
Additional EGFR Products
Product Specific Notices for Human EGFR F(ab')2 (Research Grade Cetuximab Biosimilar) Antibody
For research use only
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