Human ECE-2 Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB16451AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 233914
Product Specifications
Specificity
Detects human ECE-2 in direct ELISAs and Western blots. In Western blots, 30% cross-reactivity with recombinant human (rh) ECE‑1 and no cross-reactivity with rhKELL is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Human ECE-2 Alexa Fluor™ Plus 488-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Background: ECE-2
ECE-2 is a zinc protease of the neprilysin (NEP) family, which also includes ECE-1, PEX, XCE, DINE, Kell and several NEP-like proteins (1). ECE-2 is a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Three alternatively spliced forms differ in their cytoplasmic tail (2). In addition to big endothelin-1, ECE-2 cleaves a variety of bioactive peptides such as bradykinin, neurotensin, angiotensin I, substance P, dynorphin B, proenkephalin-derived peptides such as peptide E, BAM 22 and BAM18, and PEN-LEN, an endogenous inhibitor of prohormone convertase 1 (3). Together with ECE-1, it is also involved in degradation of beta-amyloid peptide (4). The ectodomain of human ECE-2 was expressed with an N-terminal His tag and purified.
References
- Turner, A.J. et al. (2001) BioEssays 23:261.
- Lorenzo, M.N. et al. (2001) Biochim. Biophys. Acta 1522:46.
- Mzhavia, N. et al. (2003) J. Biol. Chem. 278:14704.
- Eckman, E.A. et al. (2003) J. Biol. Chem. 278:2081.
Long Name
Endothelin-converting Enzyme-2
Alternate Names
ECE2
Entrez Gene IDs
9718 (Human)
Gene Symbol
ECE2
UniProt
Additional ECE-2 Products
Product Specific Notices for Human ECE-2 Alexa Fluor™ Plus 488-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
Loading...
Loading...
Loading...
Loading...