E6AP/UBE3A was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human E6AP/UBE3A Monoclonal Antibody (Catalog # MAB11546) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of E6AP/UBE3A in A172 Human Cell Line.

E6AP/UBE3A was detected in fixed A172 human glioblastoma cell line using Mouse Anti-Human E6AP/UBE3A Monoclonal Antibody (Catalog # MAB11546) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm and nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human E6AP/UBE3A Antibody

Application

Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: fixed A172 human glioblastoma cell line

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Formulation, Preparation, and Storage

Reconstitution

Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: E6AP/UBE3A

Ubiquitin-protein ligase E3A, also known as E6AP, is an E3 ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several key substrates for UBE3A have been identified, including BMAL1, the PML tumor suppressor, PGR, and p53/TP53 suggesting a role for UBE3A in regulation of the circadian clock, tumor regulation, transcriptional coactivation of the progesterone receptors, and regulation of neoplastic progression of cells infected by high-risk human papilloma virus (1-4). Defects in activity are linked to Angelman syndrome, a neurodevelopmental disorder, as well as autism spectrum disorders implicating a role for UBE3A in regulation of neurobiological functions (3, 5, 6).

References

Dhananjayan, S.C. et. al. (2006) Mol. Endocrinol. 20:2343.
Louria-Hayon, I. et. al. (2009) Cell Death Differ. 16:1156.
Martinez-Noel, G. et. al. (2012) Mol. Cell. Biol. 32:3095.
Gossan, N.C. et. al. (2014) Nucleic Acids Res. 42:5765.
Sadikovic, B. et. al. (2014) Hum. Mutat. 35:1407.
Khatri, N. and H-Y Man. (2019) Front. Mol. Neurosci. 12:109.

Long Name

Human Papillomavirus E6-associated Protein

Alternate Names

ANCR, EPVE6AP, HPVE6A, UBE3A

Entrez Gene IDs

7337 (Human); 22215 (Mouse); 361585 (Rat)

Gene Symbol

UBE3A

UniProt

Q05086 (Human)

Additional E6AP/UBE3A Products

Product Documents for Human E6AP/UBE3A Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human E6AP/UBE3A Antibody

For research use only

Citations
Reviews

Human E6AP/UBE3A Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11546

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human E6AP/UBE3A Antibody

Detection of E6AP/UBE3A in Human Liver.