Detects human E2F-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human E2F-2, -3, -4, -5, -6, -7, and -8 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human E2F-1 Antibody

E2F-1 in Human Placenta.

E2F-1 was detected in immersion fixed paraffin-embedded sections of human placenta using 5 µg/mL Sheep Anti-Human E2F-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4825) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human E2F-1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human skin tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human E2F-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4825) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for E2F-1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human E2F-1 Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and human skin tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E2F-1

E2F-1 (adenovirus E2 gene promoter region binding factor 1; also retinoblastoma [RB]-binding protein 3/RBP3) is a 57 - 60 kDa nuclear member of the E2F/DP family of transcription factors. It has a dual function, mediating both cell proliferation and apoptosis in a context-dependent manner. Human E2F-1 is 437 amino acids (aa) in length. It contains a cyclin A: CDK2 binding site (aa 67 - 108), a DNA-binding region (aa 110 - 194), and an RB protein binding motif (aa 409 - 426) that, when engaged, inactivates E2F-1. There is one potential in-frame 23 aa substitution for aa 89 - 111. Over aa 264 - 437, human E2F-1 shows 89% and 80% aa identity to canine and mouse E2F-1, respectively.

Long Name

E2F Transcription Factor 1

Alternate Names

E2F1, PBR3, RBAP-1, RBBP-3, RBP3

Entrez Gene IDs

1869 (Human); 13555 (Mouse); 399489 (Rat)

Gene Symbol

E2F1

UniProt

Q01094

Additional E2F-1 Products

Product Documents for Human E2F-1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human E2F-1 Antibody

For research use only

Citations
Reviews

Human E2F-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4825

Key Product Details

Species Reactivity

Validated:

Cited:

Applications