DPP6 was detected in immersion fixed paraffin-embedded sections of human spinal cord using Goat Anti-Human DPP6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2360) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of DPP6 in Human SHSY5Y Cell Line by Flow Cytometry.

Human SHSY5Y neuroblastoma cell line was stained with Goat Anti-Human DPP6 Polyclonal Antibody (Catalog # AF2360, filled histogram) or control antibody (AB-108-C, open histogram), followed by APC-conjugated Anti-Goat IgG Secondary Antibody (F0108). Staining was performed using our Staining Membrane Proteins protocol.

Applications for Human DPP6 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: SHSY5Y human neuroblastoma cell line or human differentiated neuron progenitor cells

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spinal cord

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human DPP6, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human DPP6

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPP6

Dipeptidyl peptidase IV (DPPIV/CD26) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of proteins and peptides. It plays an important role in T-cell costimulation, chemokine biology, type II diabetes and tumor biology. There are at least 15 DPPIV-related human genes. Some function as enzymes, including DPP8, DPP9, fibroblast activation protein (FAP), prolyl endopeptidase (PREP), and N-acylaminoacyl-peptide hydrolase (APEH) while others (DPP6/DPPX and DPP10/DPRP3) may have lost their protease properties but acquired novel functions. For example, DPP6 is a critical component of neuronal A-type K+ channels.

Long Name

Dipeptidyl-peptidase 6

Alternate Names

DPPX

Entrez Gene IDs

1804 (Human)

Gene Symbol

DPP6

UniProt

AAA35761

Additional DPP6 Products

Product Documents for Human DPP6 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human DPP6 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human DPP6 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2360

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all DPP6 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human DPP6 Antibody

DPP6 in Human Spinal Cord.