Human Dopamine D1R/DRD1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8276
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Saimiri sciureus (Common Squirrel Monkey)
Applications
Validated:
Immunohistochemistry, Flow Cytometry, CyTOF-ready
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 887438
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human Dopamine D1 R/DRD1
Met1-Thr446
Accession # P21728
Met1-Thr446
Accession # P21728
Specificity
Stains human Dopamine D1 R/DRD1 transfected cells but not irrelevant transfectants in flow cytometry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Dopamine D1R/DRD1 Antibody
Detection of Dopamine D1 R/DRD1 in HEK293 Human Cell Line Transfected with Human Dopamine D1/DRD1 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human Dopamine D1/DRD1 and eGFP was stained with either (A) Mouse Anti-Human Dopamine D1 R/DRD1 Monoclonal Antibody (Catalog # MAB8276) or (B) Mouse IgG2AIsotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Dopamine D1 R/DRD1 in Human Brain.
Dopamine D1 R/DRD1 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Mouse Anti-Human Dopamine D1 R/DRD1 Monoclonal Antibody (Catalog # MAB8276) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Dopamine D1R/DRD1 by Immunocytochemistry/ Immunofluorescence
Expression of MCT8 and OATP1C1 in D1-MSN (direct pathway medium-sized spiny neurons) in human and macaque striatum. Representative confocal microscope compositions from double-stained sections for MCT8 (green) or OATP1C1 (green) and for the D1-MSN marker DRD1 (red) in caudate nucleus or putamen. Merged images (right side) show the colocalization of both signals. Coexpression of MCT8 and DRD1 is observed in human (A–C) and macaque (D–F) striatum. Coexpression of OATP1C1 and DRD1 is observed in human (G–I) and macaque (J–L) striatum. Counterstaining with DAPI (blue) shows nuclei of all cells. Note that in humans the MCT8 signal is located mainly at the cell membrane, while in macaques it is located at the membrane and in the cytoplasm. Cd: caudate nucleus; DRD1: Dopamine receptor type 1; D1-MSN: D1 receptor-expressing medium-sized spiny neurons; Put: putamen. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37298594), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human Dopamine D1R/DRD1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human Dopamine D1/DRD1 and eGFP
Sample: HEK293 human embryonic kidney cell line transfected with human Dopamine D1/DRD1 and eGFP
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (caudate nucleus)
Sample: Immersion fixed paraffin-embedded sections of human brain (caudate nucleus)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dopamine D1R/DRD1
Dopamine Receptor D1 (DRD1) is a 50 kDa member of class 1 GPCR superfamily and is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. DRD1 is associated with nicotine dependence, schizophrenia, and systolic blood pressure levels.
Long Name
Dopamine D1 Receptor
Alternate Names
DADR, Dopamine D1 R, DRD1
Gene Symbol
DRD1
UniProt
Additional Dopamine D1R/DRD1 Products
Product Documents for Human Dopamine D1R/DRD1 Antibody
Product Specific Notices for Human Dopamine D1R/DRD1 Antibody
For research use only
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