Human DISC1 Alexa Fluor® 700-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6699N
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human DISC1
Lys101-Arg260
Accession # Q9NRI5
Lys101-Arg260
Accession # Q9NRI5
Specificity
Detects human DISC1 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human DISC1 Alexa Fluor® 700-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: DISC1
DISC1 (Disrupted in Schizophrenia 1) is a 100-105 kDa cytoplasmic and mitochondrial protein that belongs to no known molecular family. It is widely expressed, and appears to have multiple interaction partners, among which are NDEL1, alpha-tubulin, TRAF3IP1 and GSK-3 beta. DISC1 is of particular interest in the brain where it appears to play a role in both neuronal proliferation and migration. Regarding proliferation, DISC1 inhibits GSK-3 beta activity, resulting in neural progenitor cell proliferation without differentiation. With respect to migration, DISC1 promotes embryonic subventricular neuron migration while inhibiting widespread adult neuronal migration from the hippocampal subgranular layer. Human DISC1 is 854 amino acids (aa) in length. It contains an N-terminal globular domain (aa 1-346) plus four coiled-coil regions (aa 366‑830). DISC1 is phosphorylated and forms homodimers. There are multiple isoforms. Among them is a 65-70 kDa form that shows an 18 aa substitution for aa 661-854, a 48 kDa form that contains a 20 aa substitution for aa 350-854, a 61-64 kDa form that possesses a seven aa substitution for aa 545-854, and a 90 kDa form that contains a deletion of aa 748-769. Over aa 101-260, human DISC1 shares 44% aa identity with mouse DISC1.
Long Name
Disrupted in Schizophrenia 1
Alternate Names
C1orf136, SCZD9
Gene Symbol
DISC1
UniProt
Additional DISC1 Products
Product Specific Notices for Human DISC1 Alexa Fluor® 700-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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