Human Dermatopontin Alexa Fluor™ Plus 680-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4629AFP680
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Dermatopontin
Specificity
Detects human Dermatopontin in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Dermatopontin is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Dermatopontin
Dermatopontin, also known as TRAMP (tyrosine rich acidic matrix protein), is a widely expressed noncollagenous protein component of the extracellular matrix (1, 2). Mature human Dermatopontin shares 96%, 92%, and 92% amino acid sequence identity with bovine, mouse, and rat Dermatopontin, respectively. It is a 22 kDa molecule that is tyrosine sulfated but not glycosylated (3, 4). Dermatopontin contains three disulfide bonded loop structures that enclose conserved hexapeptide motifs (5). It accelerates collagen fibril formation in vitro, and Dermatopontin deficient mice exhibit altered collagen matrix deposition and organization (6 - 8). Dermatopontin is downregulated in fibrotic growths such as leiomyoma and scar tissue (9, 10). It binds both TGF-beta and the proteoglycan decorin, interactions that can increase the bioavailability of TGF-beta (11, 12). Dermatopontin promotes bone mineralization under the control of the vitamin D receptor and inhibits BMP-2 effects on osteoblast precursors (13, 14).
References
- Okamoto, O. and S. Fujiwara (2006) Connect. Tissue Res. 47:177.
- Superti-Furga, A. et al. (1993) Genomics 17:463.
- Forbes, E.G. et al. (1994) FEBS Lett. 351:433.
- Cronshaw, A.D. et al. (1993) Matrix 13:255.
- Neame, P.J. et al. (1989) J. Biol. Chem. 264:5474.
- MacBeath, J.R.E. et al. (1993) J. Biol. Chem. 268:19826.
- Takeda, U. et al. (2002) J. Invest. Dermatol. 119:678.
- Cooper, L.J. et al. (2006) Invest. Opthalmol. Vis. Sci. 47:3303.
- Catherino, W.H. et al. (2004) Genes Chromosomes Cancer 40:204.
- Kuroda, K. et al. (1999) J. Invest. Dermatol. 112:706.
- Okamoto, O. et al. (1996) J. Biochem. 119:106.
- Okamoto, O. et al. (1999) Biochem. J. 337:537.
- Pochampally, R.R. et al. (2007) J. Bone Miner. Res. 22:1338.
- Behnam, K. et al. (2006) Connect. Tissue Res. 47:271.
Alternate Names
DPT, Eq1
Gene Symbol
DPT
UniProt
Additional Dermatopontin Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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