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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Bovine

Applications

CyTOF-ready, Flow Cytometry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 143-41

Product Summary for Human DEP-1/CD148 Antibody

Immunogen

Phytohemagglutinin-stimulated human peripheral blood mononuclear cells

Specificity

Detects human DEP-1/CD148 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human DEP-1/CD148 Antibody

Detection of Human DEP-1/CD148 antibody by Western Blot.

Detection of Human DEP-1/CD148 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human DEP-1/CD148 Monoclonal Antibody (Catalog # MAB1934) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DEP-1/CD148 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human DEP-1/CD148 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood lymphocytes

Immunoprecipitation

2.5 µg/500 µg cell lysate
Sample: HeLa human cervical epithelial carcinoma cell line, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and Raji human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB1934 in the following applications:

Published Applications

Read 5 publications using MAB1934 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DEP-1/CD148

Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kDa) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T‑cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta-catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.

References

  1. Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680.
  2. Sorby, M. et al. (2001) Oncogene 20:5219.
  3. Jandt, E. et al. (2003) Oncogene 22:4175.
  4. Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793.
  5. Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393.
  6. Borges, L.G. et al. (1996) Circ. Res. 79:570.
  7. de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800.
  8. Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295.
  9. Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817.
  10. Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728.
  11. Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219.
  12. Holsinger, L.J. et al. (2002) Oncogene 21:7067.

Long Name

Density Enhanced Receptor Protein Tyrosine Phosphatase

Alternate Names

CD148, DEP1, HPTP-eta, PTPRJ

Entrez Gene IDs

5795 (Human); 19271 (Mouse); 29645 (Rat)

Gene Symbol

PTPRJ

Additional DEP-1/CD148 Products

Product Documents for Human DEP-1/CD148 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human DEP-1/CD148 Antibody

For research use only

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