Human DEP-1/CD148 Antibody

Name: Human DEP-1/CD148 Antibody
Brand: R&D Systems
SKU: MAB1934

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB1934

Citations (5)

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Species Reactivity:	Human
Immunogen:	Phytohemagglutinin-stimulated human peripheral blood mononuclear cells
Applications:	CyTOF-ready, Flow Cytometry, Immunoprecipitation, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 143-41
Specificity:	Detects human DEP-1/CD148 in Western blots.

View Full Technical Details for Human DEP-1/CD148 Antibody

Applications for Human DEP-1/CD148 Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Flow Cytometry	2.5 µg/10⁶ cells	Human peripheral blood lymphocytes
Immunoprecipitation	2.5 µg/500 µg cell lysate	HeLa human cervical epithelial carcinoma cell line, see our available Western blot detection antibodies
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human DEP-1/CD148 Antibody

	Western Blot Detection of Human DEP‑1/CD148 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human DEP-1/CD148 Monoclonal Antibody (Catalog # MAB1934) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DEP-1/CD148 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: DEP-1/CD148

Density Enhanced Protein Tyrosine Phosphatase (DEP-1), also known as CD148, HPTP-eta, and PTP receptor type J (PTPRJ), is an enzyme that removes phosphate groups covalently attached to tyrosine residues in proteins. A large (220 kDa) glycoprotein found at the cell surface, DEP-1 levels are increased with high cell density (1). DEP-1 phosphatase activity is enhanced by basement membrane proteins (2), suggesting it is involved in regulating cell adhesion and contact interactions. High levels of expression dampen PDGF (3), VEGF (4), and T‑cell receptor (5) responses. DEP-1 is widely expressed in tissues, particularly ones forming epithelioid monolayers (6). In the immune system, DEP-1 is found on all cell lineages and is highest on granulocytes (7). Dep-1 is the mutated gene in the Susceptibility to Colon Cancer locus Scc1, which is altered in many human colorectal adenomas (8). Gene knockout mice lacking DEP-1 die at midgestation due to failures in cardiovascular development (9). DEP-1 dephosphorylates a variety of proteins, including the HGF (10), PDGF (11), and VEGF (4) receptors, and beta-catenin (12). The recombinant protein is the intracellular region of DEP-1 containing the catalytic domain.

Item	Value
References	Ostman, A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9680. Sorby, M. et al. (2001) Oncogene 20:5219. Jandt, E. et al. (2003) Oncogene 22:4175. Lampugnani, M.G. et al. (2003) J. Cell Biol. 161:793. Baker, J.E. et al. (2001) Mol. Cell. Biol. 21:2393. Borges, L.G. et al. (1996) Circ. Res. 79:570. de la Fuente-Garcia, M.A. et al. (1998) Blood 91:2800. Ruivenkamp, C.A. et al. (2002) Nat. Genet. 31:295. Takahashi, T. et al. (2003) Mol. Cell. Biol. 23:1817. Palka, H.L. et al. (2003) J. Biol. Chem. 278:5728. Kovalenko, M. et al. (2000) J. Biol. Chem. 275:16219. Holsinger, L.J. et al. (2002) Oncogene 21:7067.
Long Name	Density Enhanced Receptor Protein Tyrosine Phosphatase
Entrez Gene IDs	5795 (Human); 19271 (Mouse); 29645 (Rat)
Alternate Names	CD148, CD148 antigen, DEP-1, DEP1, DEP1EC 3.1.3.48, Density-enhanced phosphatase 1, HPTP eta, HPTP-eta, HPTPeta, PTPRJ, Protein-tyrosine phosphatase eta, Protein-tyrosine phosphatase receptor type J, R-PTP-ETA, R-PTP-J, SCC1, human density enhanced phosphatase-1, protein tyrosine phosphatase, receptor type, J, protein tyrosine phosphatase, receptor type, J polypeptide, receptor-type tyrosine-protein phosphatase eta, susceptibility to colon cancer 1, mouse, homolog of

Citations for Human DEP-1/CD148 Antibody (5)

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Citations are publications that use Bio-Techne products. Selected citations for Human DEP-1/CD148 Antibody include:

Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

A Bilotta et al. (2016), A novel splice variant of the protein tyrosine phosphatase PTPRJ that encodes for a soluble protein involved in angiogenesis Oncotarget, 0(0).
PMID: 28052032
Spring K et al. (2015), The protein tyrosine phosphatase DEP-1/PTPRJ promotes breast cancer cell invasion and metastasis. Oncogene, 34(44):5536-47.
PMID: 25772245
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Spring K et al. (2014), Phosphorylation of DEP-1/PTPRJ on threonine 1318 regulates Src activation and endothelial cell permeability induced by vascular endothelial growth factor. Cell Signal, 26(6):1283-93.
PMID: 24583284
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Cabezon R et al. (2011), Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. Immunol. Lett., 134(2):167-73.
PMID: 20933012
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Ambarus C et al. (0), Systematic validation of specific phenotypic markers for in vitro polarized human macrophages. J Immunol Method375(1):196-206.
PMID: 22075274

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Human DEP-1/CD148 Antibody

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Scientific Data Examples for Human DEP-1/CD148 Antibody

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