Human DC-SIGN+DC-SIGNR PE-conjugated Antibody

Name: Human DC-SIGN+DC-SIGNR PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB1621P

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB1621P

Citations (2)

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Key Product Details

Species Reactivity:	Human
Immunogen:	NIH-3T3 mouse embryonic fibroblast cell line transfected with human DC-SIGNR Accession # Q9H2X3
Applications:	Flow Cytometry
Label:	Phycoerythrin (Excitation=488 nm, Emission=565-605 nm)
Source:	Monoclonal Mouse IgG_2A Clone # 120612
Specificity:	Recognizes both human DC-SIGN and human DC-SIGNR on transfected cells. Does not react with parental mouse cells or irrelevant transfectants.

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Applications for Human DC-SIGN+DC-SIGNR PE-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human DC-SIGN+DC-SIGNR PE-conjugated Antibody

	Flow Cytometry Detection of DC‑SIGN+DC‑SIGNR in NIH‑3T3 Mouse Cell Line Transfected with Human DC-SIGN and DC-SIGNR by Flow Cytometry. NIH-3T3 mouse embryonic fibroblast cell line transfected with (A) human DC-SIGN and (B) human DC-SIGNR was stained with Mouse Anti-Human DC-SIGN+DC-SIGNR PE-conjugated Monoclonal Antibody (Catalog # FAB1621P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Background: DC-SIGN+DC-SIGNR

DC-SIGN (Dendritic Cell-Specific ICAM-3 Grabbing Non-integrin) has been shown to play an important role in regulating Dendritic Cell (DC) and T cell interactions, including antigen presentation to T cells and enhancement of transinfection of CD4+ T cells by HIV-1 (1, 2). Efforts to identify additional type II membrane proteins resulted in the isolation of a molecule related in sequence to DC-SIGN known as DC-SIGNR (DC-SIGN Related) (3, 4). DC-SIGNR shares 73 - 80% amino acid homology with DC-SIGN and is located on human chromosome 19p13.3. Its structure is similar to DC-SIGN and therefore binds mannose residues in a calcium dependent fashion, including ICAM-3 and HIV-1 gp120 (5). DC-SIGNR, also known as L-SIGN (Liver/Lymph node-Specific ICAM-3-Grabbing Non-integrin) and DC‑SIGNR, is polymorphic since allelic variations of the exon 4 encoded sequence have been isolated (5). This is further supported by a study demonstrating the ability to isolate a large repertoire of DC-SIGNR transcripts largely the result of alternative splicing of the 7 coding exons (6). L-SIGN/DC-SIGNR is primarily transcribed in the liver and lymph nodes but not in monocyte derived DC (5). Expression of L-SIGN/DC-SIGNR is restricted to endothelial cells derived from liver sinusoids, lymph node sinuses and capillaries (7) although variable expression in placenta and some monocytic cell lines has also been reported, including both membrane and soluble isoforms of the protein (6). Expression of DC-SIGN is induced during the in vitro generation of DC from either monocytes or bone marrow progenitors, with maximal surface expression at day 7 of culture (1). Immature DC in the skin and mature DC in the tonsil have been demonstrated to express DC‑SIGN (8). Analysis of various tissues and cell lines suggests that DC-SIGN expression is restricted to DC (1) although a more recent report finds evidence of expression in placenta, resting monocytes and monocytic cell lines (6). This discrepancy may be partially related to the multiple isoforms of DC-SIGN transcripts, including both membrane and soluble forms, as well as exon splice variants reported in the latter study (6).

Item	Value
References	Geijtenbeek, T.B.H. et al. (2000) Cell 100:575. Geijtenbeek, T.B.H. et al. (2000) Cell 100:587. Yokoyama-Kobayashi, M.T. et al. (1999) Gene 228:161. Soilleux, E.J. et al. (2000) J. Immunol. 165:2937. Bashirova, A.A. et al. (2001) J. Exp. Med. 193:671. Mummidi, S. et al. (2001) J. Biol. Chem. 276:33196.. Pohlman, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:2670. Geijtenbeek, T.B.H. et al. (2000) Nature Immunol. 1:353.
Long Name	Dendritic Cell-specific ICAM-3-grabbing Non-integrin
Entrez Gene IDs	30835 (Human)
Alternate Names	DC-SIGN+DC-SIGNR, DCSIGN+DCSIGNR

Citations for Human DC-SIGN+DC-SIGNR PE-conjugated Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human DC-SIGN+DC-SIGNR PE-conjugated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

T Suzuki et al. (2020), Severe fever with thrombocytopenia syndrome virus targets B cells in lethal human infections J. Clin. Invest., 130(2):799-812.
PMID: 31904586
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Meier R et al. (2014), Genome-wide small interfering RNA screens reveal VAMP3 as a novel host factor required for Uukuniemi virus late penetration. J Virol, 88(15):8565-78.
PMID: 24850728

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