Human Cytosolic beta-Glucosidase/GBA3 Alexa Fluor™ Plus 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5969AFP405
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cytosolic beta‑Glucosidase/GBA3
Specificity
Detects human Cytosolic beta‑Glucosidase/GBA3 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Cytosolic beta-Glucosidase/GBA3
There are three beta‑glucosidases (GBA) in human genome. GBA1 endodes a lysosomal membrane protein that cleaves the beta‑glucosidic linkage of glucosylceramide (1). GBA2 encodes a microsomal beta-glucosidase that catalyzes the hydrolysis of bile acid 3-O-glucosides (2). GBA3 is a cytosolic beta‑glucosidase and is predominantly expressed in liver. GBA3 efficiently hydrolyzes beta‑D‑glucoside and beta‑D‑galactoside, but not any known physiological beta‑glycoside, suggesting that it may be involved in detoxification of plant glycosides (3). GBA3 also has significant neutral glycosylceramidase activity, suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (4). At the protein level, GBA3 shows significant homology (>40%) with Klotho protein that is known for its association with aging process (3, 4).
References
- Tybulewicz, V.L. et al. (1992) Nature 357:407.
- Matern, H. et al. (2001) J. Biol. Chem. 276:37929.
- de Graaf, M. et al. (2001) Biochem. J. 356:907.
- Hayashi, Y. et al. (2007) J. Biol. Chem. 282:30889.
Alternate Names
CBGL1, Cytosolic betaGlucosidase, GBA3, GLUC, KLrP
Gene Symbol
GBA3
UniProt
Additional Cytosolic beta-Glucosidase/GBA3 Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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