Detects human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human CPGL-2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL (Catalog # 3560-ZN), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL (Catalog # 3560-ZN)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL

The human CNDP2 gene encodes cytosol non-specific dipeptidase, a member of the M20 family of metalloproteases (1, 2). Also known as glutamate carboxypeptidase-like protein 1 (CPGL), tissue carnosinase or peptidase A, CNDP2 is ubiquitously expressed throughout human tissues. Unlike CNDP1, CNDP2 has broad substrate specificity for dipeptides with free amino and carboxyl groups. The purified recombinant human CNDP2 migrates as three bands in SDS-PAGE under reducing conditions that correspond to the full length protein (54 kDa), the N-terminal fragment (33 kDa) and the C-terminal fragment (22 kDa). The initiator Met1 may be removed and Ala2 may be acetylated based on Accession # Q96KP4.

References

Teufel, M. et al. (2003) J. Biol. Chem. 278:6521.
Woessner, J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp.1020, Academic Press, San Diego.

Alternate Names

CN2, CNDP2, CPGL, PEPA

Entrez Gene IDs

55748 (Human)

Gene Symbol

CNDP2

UniProt

Q96KP4

Additional Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL Products

Product Documents for Human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL Antibody

For research use only

Citations
Reviews

Human Cytosol Nonspecific Dipeptidase (CNDP2)/CPGL Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3560

Key Product Details

Species Reactivity

Validated:

Cited:

Applications