Human Cystatin A Alexa Fluor™ Plus 405-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1407AFP405
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Cystatin A
Specificity
Detects Cystatin A in direct ELISAs and Western blots. In Western blots, less than 1% cross‑reactivity with recombinant mouse (rm) Cystatin A, recombinant human (rh) Cystatin B, rhCystatin C, rhCystatin D, rhCystatin E/M, rhCystatin F, rhCystatin S, rhCystatin SA and rhCystatin SN is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Immunoprecipitation
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Cystatin A
Cystatin A, also called stefin A, Cystatin AS or keratolinin, is a member of family 1 of the cystatin superfamily (1, 2). Like Cystatin B, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (3). For example, immunohistochemical analysis of Cystatin A and cathepsin L is a useful indicator for malignancy in human epidermal keratinocytes (4). The ratio of cathepsin B and Cystatin A can be used in the differential diagnosis and treatment of patients with prostate carcinoma (5). The human Cystatin A consists of 98 amino acids (1).
References
- Kartasova, T. et al. (1987) Nucleic Acids Res. 15:5945.
- Abrahamson, M. (1994) Methods Enzymol. 244:685.
- Jenko, S. et al. (2003) J. Mol. Biol. 326:875.
- Palungwachira, P. et al. (2002) J. Dermatol. 29:573.
- Sinha, A.A. et al. (2002) Cancer 94:3141.
Alternate Names
AREI, CSTA, Stefin A, STF1, STFA
Entrez Gene IDs
1475 (Human)
Gene Symbol
CSTA
UniProt
Additional Cystatin A Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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