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Human Cyr61/CCN1 Antibody

Catalog # MAB40551 | R&D Systems, Inc. a Bio-Techne Brand
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MAB40551-100
MAB40551-SP

Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 434311

Product Summary for Human Cyr61/CCN1 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cyr61/CCN1
Ala22-Asp381
Accession # O00622

Specificity

Detects human Cyr61/CCN1 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Cyr61/CCN1 Antibody

Human Cyr61/CCN1 Antibody in ELISA Standard Curve.

Human Cyr61/CCN1 ELISA Standard Curve.

Recombinant Human Cyr61/CCN1 protein was serially diluted 2-fold and captured by Rat Anti-Human Cyr61/CCN1 Monoclonal Antibody (Catalog # MAB40551) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6009) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human Cyr61/CCN1 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6009).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Cyr61/CCN1 DuoSet ELISA Kit (Catalog # DY4055) for convenient development of a sandwich ELISA or the Human Cyr61/CCN1 Quantikine ELISA Kit (Catalog # DCYR10) for a complete optimized ELISA.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cyr61/CCN1

Cyr61, also known as CCN1, is a 40 - 45 kDa matricellular glycoprotein that plays an important role in cellular adhesion and migration (1). Cyr61 consists of an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain (2). Mature human Cyr61 shares 93% amino acid sequence identity with mouse and rat Cyr61. It is widely expressed during development and in adult tissues (2, 3). Cyr61 associates with the extracellular matrix (ECM) and with many cell surface molecules including Integrins alphaV beta3, alphaV beta5, alphaM beta2, and alpha6 beta1, Syndecan-4, and heparan sulfate proteoglycans (1, 3). Cyr61 mediates the adhesion and migration of multiple cell types and also promotes vascular endothelial cell tubule formation (4 - 6). Plasmin cleavage of ECM-bound Cyr61 releases a 28 kDa N-terminal fragment which retains the ability to promote endothelial cell migration (7). Cyr61 exhibits both tumorigenic and tumor suppressor properties. It is upregulated and promotes tumorigenesis, angiogenesis, and metastasis in breast, renal, gastric, squamous cell, and colorectal carcinomas as well as in glioma (8 - 12). In contrast, when downregulated, it suppresses tumor growth in endometrial, hepatic, and non-small cell lung cancers (8, 13, 14). Cyr61 is also upregulated in injured skin and bone where it induces the expression of growth factors, cytokines, proteases, and integrins involved in wound repair (15, 16).

References

  1. Chen, Y. and X.Y. Du (2007) J. Cell. Biochem. 100:1337.
  2. Jay, P. et al. (1997) Oncogene 14:1753.
  3. Feng, P. et al. (2008) Int. J. Biochem. Cell Biol. 40:98.
  4. Chen, N. et al. (2000) J. Biol. Chem. 275:24953.
  5. Leu, S-J. et al. (2002) J. Biol. Chem. 277:46248.
  6. Schutze, N. et al. (2007) BMC Cell Biol. 8:45.
  7. Pendurthi, U.R. et al. (2005) Cancer Res. 65:9705.
  8. O'Kelly, J. et al. (2008) Int. J. Oncol. 33:59.
  9. Chintalapudi, M.R. et al. (2008) Carcinogenesis 29:696.
  10. Xie, D. et al. (2004) Cancer Res. 64:1987.
  11. Lin, M-T. et al. (2007) J. Biol. Chem. 282:34594.
  12. Monnier, Y. et al. (2008) Cancer Res. 68:7323.
  13. Tong, X. et al. (2001) J. Biol. Chem. 276:47709.
  14. Chien, W. et al. (2004) J. Biol. Chem. 279:53087.
  15. Hadjiargyrou, M. et al. (2000) J. Bone Miner. Res.15:1014.
  16. Chen, C-C. et al. (2001) J. Biol. Chem. 276:47329.

Long Name

Cysteine-Rich, Angiogenic Inducer 61

Alternate Names

CCN1, GIG1, IGFBP-10

Entrez Gene IDs

3491 (Human); 16007 (Mouse); 83476 (Rat)

Gene Symbol

CCN1

UniProt

Additional Cyr61/CCN1 Products

Product Documents for Human Cyr61/CCN1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Cyr61/CCN1 Antibody

For research use only

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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