HEK293 human embryonic kidney cell line transfected with human CXCR6 was stained with Mouse Anti-Human CXCR6 APC-conjugated Monoclonal Antibody (Catalog # FAB699A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of CXCR6 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 9 days were stained with Mouse Anti-Human CXCR6 APC-conjugated Monoclonal Antibody (Catalog # FAB699A) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Detection of CXCR6 by Flow Cytometry

CXCR6 promotes BC cells migration and invasionA) CXCR6 was overexpressed in MCF-7 and MDA-231 BC cells by lentiviral system. CXCR6 -positive cells were then sorted by flow cytometry. Histogram displays the ratio of CXCR6 positive expression of MCF-7CXCR6, MDA-231CXCR6 and their respective control cells. B) Scratch wound healing assay shows that overexpression of CXCR6 enhanced cell migration in MDA-231 cells, but not very significant in MCF-7 cells. C) Matrigel invasion assay showed that overexpression of CXCR6 significantly enhanced cell invasion in both MCF-7 and MDA-231 cells (***P < 0.001). Experiments were performed 3 times; representative images of invaded cells are shown. Mean ± SD. D) Cytometry analysis showed CXCR6 had no effect on cell cycle. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25909173), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CXCR6 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human CXCR6 and human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human IL‑2 (Catalog # 202-IL)

Reviewed Applications

Read 4 reviews rated 4.5 using FAB699A in the following applications:

Flow Cytometry (4 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR6

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

Alternate Names

Bonzo, CD186, CXCR6, STRL33

Entrez Gene IDs

10663 (Human); 80901 (Mouse); 100124593 (Rat)

Gene Symbol

CXCR6

UniProt

O00574

Additional CXCR6 Products

Product Documents for Human CXCR6 APC-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human CXCR6 APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human CXCR6 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB699A

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CXCR6 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CXCR6 APC-conjugated Antibody

Detection of CXCR6 in HEK293 Human Cell Line Transfected with Human CXCR6 by Flow Cytometry.