Sign In / Register
Human CXCR5 Antibody
Catalog # MAB190 | R&D Systems, Inc. a Bio-Techne Brand
Clone 51505 was used by HLDA to establish CD designation
Key Product Details
Species Reactivity
Human
Applications
CyTOF-reported, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 51505
Customers Also Viewed
Product Summary for Human CXCR5 Antibody
Immunogen
NS0 mouse myeloma cell line transfected with human CXCR5
Met1-Phe372
Accession # P32302
Met1-Phe372
Accession # P32302
Specificity
Stains human CXCR5 transfectants but not the parental cell lines in flow cytometry. Does not cross-react with human CXCR2, CXCR3, or CXCR4 transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human CXCR5 Antibody
Detection of CXCR5 in CD19+ Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) or (B) Mouse IgG2B control antibody (Catalog # MAB0041) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
CXCR5 in Human PBMCs.
CXCR5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 637-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL008) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CXCR5 in Human Kidney.
CXCR5 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human CXCR5 Monoclonal Antibody (Catalog # MAB190) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human CXCR5 Antibody
Application
Recommended Usage
CyTOF-reported
Ferrell, P.B., Jr. et al. (2016) PLoS ONE 11: e0153207. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human peripheral blood CD19+ B cells
Sample: Human peripheral blood CD19+ B cells
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Sample: Immersion fixed human peripheral blood mononuclear cells (PBMCs)
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node and human kidney
Sample: Immersion fixed paraffin-embedded sections of human lymph node and human kidney
Neutralization
Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND50) is typically 0.25-1.5 µg/mL in the presence of 0.05 µg/mL Recombinant Human CXCL13/BLC/BCA‑1.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using MAB190 in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR5
Product Documents for Human CXCR5 Antibody
Product Specific Notices for Human CXCR5 Antibody
For research use only
Citations for Human CXCR5 Antibody (27)
Showing
1
-
10 of
27 citations
Showing All
Filter By:
Search Title:
Citations are publications that use Bio-Techne products. Selected citations for Human CXCR5 Antibody include:
-
Species: Human
Sample Types: Whole Tissue
Applications: IHCN Kaneko et al. (2022-09-14), Cytotoxic CD8+ T cells may be drivers of tissue destruction in Sj�gren's syndrome Scientific Reports, 2022-012(1):15427.
PMID: 36104369 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCA Julià et al. (2020-08-25), Lower peripheral helper T cell levels in the synovium are associated with a better response to anti-TNF therapy in rheumatoid arthritis Arthritis Res. Ther., 2020-022(1):196.
PMID: 32843099 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryF Facciotti et al. (2020-03-17), Evidence for a pathogenic role of extrafollicular, IL-10-producing CCR6+B helper T cells in systemic lupus erythematosus Proc. Natl. Acad. Sci. U.S.A., 2020-0117(13):7305-7316.
PMID: 32184325 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationE Armas-Gonz et al. (2018-06-07), Role of CXCL13 and CCL20 in the recruitment of B cells to inflammatory foci in chronic arthritis Arthritis Res. Ther., 2018-020(1):114.
PMID: 29880013 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PLA Aqrawi et al. (2018-03-24), Diminished CXCR5 expression in peripheral blood of patients with Sj�gren's syndrome may relate to both genotype and salivary gland homing Clin. Exp. Immunol., 2018-00(0).
PMID: 29453859 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotMitkin N et al. (2015-03-19), p53-dependent expression of CXCR5 chemokine receptor in MCF-7 breast cancer cells. Sci Rep, 2015-05(0):9330.
PMID: 25786345 -
Species: Human
Sample Types: Peptide
Applications: Biacore Binding AssayChu et al. (2014-12-08), Capture-stabilize approach for membrane protein SPR assays. Sci Rep, 2014-14(0):7360.
PMID: 25484112 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryRasul E et al. (2014-08-27), The MEC1 and MEC2 lines represent two CLL subclones in different stages of progression towards prolymphocytic leukemia. PLoS ONE, 2014-09(8):e106008.
PMID: 25162594 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryHumpert M et al. (2014-02-19), CXCR7 influences the migration of B cells during maturation. Eur J Immunol, 2014-044(3):694-705.
PMID: 24259140 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryMazerolles F et al. (2013-02-10), Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency. J Allergy Clin Immunol, 2013-0131(4):1146-56, 1156.
PMID: 23403044 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-FrSarra M et al. (2012-07-11), IL-15 positively regulates IL-21 production in celiac disease mucosa. Mucosal Immunol, 2012-06(2):244-55.
PMID: 22785229 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryIslam SA et al. (2011-01-09), Mouse CCL8, a CCR8 agonist, promotes atopic dermatitis by recruiting IL-5+ T(H)2 cells. Nat. Immunol., 2011-012(2):167-77.
PMID: 21217759 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryWeiss N et al. (2010-04-18), IL8 and CXCL13 are potent chemokines for the recruitment of human neural precursor cells across brain endothelial cells. J Neuroimmunol, 2010-0223(1):131-4.
PMID: 20400187 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationSingh S et al. (2009-04-17), Serum CXCL13 positively correlates with prostatic disease, prostate-specific antigen and mediates prostate cancer cell invasion, integrin clustering and cell adhesion. Cancer Lett., 2009-0283(1):29-35.
PMID: 19375853 -
Species: Human
Sample Types: Tissue Homogenates,Whole Tissue
Applications: Western Blot,IHC-PPanse J et al. (2008-09-16), Chemokine CXCL13 is overexpressed in the tumour tissue and in the peripheral blood of breast cancer patients. Br. J. Cancer, 2008-099(6):930-8.
PMID: 18781150 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PCagigi A et al. (2008-09-09), Altered expression of the receptor-ligand pair CXCR5/CXCL13 in B cells during chronic HIV-1 infection. Blood, 2008-0112(12):4401-10.
PMID: 18780835 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryKang SG et al. (2007-09-15), Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells. J. Immunol., 2007-0179(6):3724-33.
PMID: 17785809 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationBurkle A et al. (2007-07-25), Overexpression of the CXCR5 chemokine receptor, and its ligand, CXCL13 in B-cell chronic lymphocytic leukemia. Blood, 2007-0110(9):3316-25.
PMID: 17652619 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryPonte AL et al. (2007-03-29), The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities. Stem Cells, 2007-025(7):1737-45.
PMID: 17395768 -
Species: Human
Sample Types: Whole Cells
Applications: ICCLisignoli G et al. (2006-05-01), Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54. J. Cell. Physiol., 2006-0207(2):364-73.
PMID: 16331675 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHCHaas CS et al. (2005-12-01), Chemokine receptor expression in rat adjuvant-induced arthritis. Arthritis Rheum., 2005-152(12):3718-30.
PMID: 16320322 -
Species: Human
Sample Types: Whole Cells
Applications: ICCLim HW et al. (2004-12-01), Regulatory T cells can migrate to follicles upon T cell activation and suppress GC-Th cells and GC-Th cell-driven B cell responses. J. Clin. Invest., 2004-1114(11):1640-9.
PMID: 15578096 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryKohrgruber N et al. (2004-12-01), Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J. Immunol., 2004-1173(11):6592-602.
PMID: 15557149 -
Species: Rabbit
Sample Types: Whole Cells
Applications: Flow CytometrySinha RK et al. (2004-06-01), Developing neonatal rabbit appendix, a primary lymphoid organ, is seeded by immature blood-borne B cells: evidence for roles for CD62L/PNAd, CCR7/CCL21, alpha4beta1 and LFA-1. Dev. Comp. Immunol., 2004-028(7):829-41.
PMID: 15043950 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryAnnels NE et al. (2003-12-04), Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL. Blood, 2003-1103(7):2806-8.
PMID: 14656893 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-FrHuber TB et al. (2002-06-15), Expression of functional CCR and CXCR chemokine receptors in podocytes. J. Immunol., 2002-0168(12):6244-52.
PMID: 12055238 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryCampbell J et al. (2001-06-01), Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire. J Immunol, 2001-0166(11):6477-82.
PMID: 11359797
There are no citations that match your criteria.
Customer Reviews for Human CXCR5 Antibody (2)
4.5 out of 5
2 customer
ratings
Customer Images
Showing
1
-
2 of
2 reviews
Showing All
Filter By:
-
Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Human retinal pigment epithelium cellsSpecies: HumanVerified Customer | Posted 08/19/2021
-
Application: ImmunohistochemistrySample Tested: Tonsil tissueSpecies: HumanVerified Customer | Posted 09/11/2020retrieval in high pH buffer Ab was diluted 1:2000(0.25ug/mL)
There are no reviews that match your criteria.
Loading...
