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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 48311

Product Summary for Human CXCR2/IL-8RB Antibody

Immunogen

NS0 mouse myeloma cell line transfected with human CXCR2/IL-8 RB
Met1-Leu355
Accession # AAB25880

Specificity

Detects human CXCR2/IL-8 RB transfected NS0 cells but not the parental cell line. It does not cross-react with CXCR1 (IL-8 RA).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human CXCR2/IL-8RB Antibody

Detection of CXCR2/IL-8 RB antibody in Human Blood Granulocytes antibody by Flow Cytometry.

Detection of CXCR2/IL‑8 RB in Human Blood Granulocytes by Flow Cytometry.

Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB331, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
CXCR2/IL‑8 RB antibody in Human Lymph Node by Immunohistochemistry (IHC-P).

CXCR2/IL‑8 RB in Human Lymph Node.

CXCR2/IL-8 RB was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Mouse Anti-Human CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB331) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Chemotaxis Induced by CXCL1/GRO alpha and Neutralization by Human CXCR2/IL‑8 RB Antibody.

Chemotaxis Induced by CXCL1/GRO alpha and Neutralization by Human CXCR2/IL‑8 RB Antibody.

Recombinant Human CXCL1/GROa (Catalog # 275-GR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL1/GROa (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB331). The ND50 is typically 1-5 µg/mL.

Applications for Human CXCR2/IL-8RB Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human whole blood granulocytes

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymph node

Neutralization

Measured by its ability to neutralize CXCL1/GRO alpha/KC/CINC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 5 ng/mL Recombinant Human CXCL1/GRO alpha/KC/CINC‑1.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 6 reviews rated 4.3 using MAB331 in the following applications:

Published Applications

Read 62 publications using MAB331 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR2/IL-8RB

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.

Long Name

Interleukin 8 Receptor B

Alternate Names

CD182, CDw128b, CMKAR2, CXCR-2, IL-8 RB, IL8RB

Entrez Gene IDs

3579 (Human); 12765 (Mouse); 29385 (Rat)

Gene Symbol

CXCR2

UniProt

Additional CXCR2/IL-8RB Products

Product Documents for Human CXCR2/IL-8RB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CXCR2/IL-8RB Antibody

For research use only

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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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