Human CTCF Alexa Fluor™ Plus 555-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6369AFP555
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CTCF
Specificity
Detects human CTCF in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human CTCF Alexa Fluor™ Plus 555-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: CTCF
CTCF (CCCTC-binding factor) is a 130‑140 kDa member of the CTCF Zn-finger protein family. Although its predicted MW is 83 kDa, it runs anomalously slow in SDS‑PAGE. CTCF is ubiquitously expressed, save for primary spermatocytes, and serves multiple functions. It regulates both gene activation and repression by binding to unmethylated DNA sequences. Structurally, it contains eleven Zn-finger motifs, some or all of which may be involved in DNA binding. This variation in number allows for different conformations and multiple interaction partners. Human CTCF is 727 amino acids (aa) in length. It contains two SUMOylation sites (Lys74 and Lys689) and eleven consecutive C2H2-type Zn-finger domains (aa 266‑577). There are multiple Ser/Thr phosphorylation sites. Two potential alternative start sites exist; one nine aa upstream of the standard start site, and another at Met570. A third splice variant shows a 62 aa substitution for aa 349‑727. Over aa 1‑154, human CTCF shares 99% aa identity with mouse CTCF.
Long Name
CCCTC-binding Factor
Alternate Names
11-Zinc Finger Protein2, CCCTC-binding Factor
Gene Symbol
CTCF
UniProt
Additional CTCF Products
Product Specific Notices for Human CTCF Alexa Fluor™ Plus 555-conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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