Human Collagen IV alpha1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11758
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Multiplex Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG Clone # 1106301
Product Specifications
Immunogen
Synthetic peptide
Accession # P02462
Accession # P02462
Specificity
Detects recombinant human COL4A1 protein in Direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Scientific Data Images for Human Collagen IV alpha1 Antibody
COL4A1 in Human Kidney via seqIF™ staining on COMET™
COL4A1 was detected in immersion fixed paraffin-embedded sections of human Kidney using Mouse Anti-Human COL4A1, Monoclonal Antibody (Catalog#MAB11758) at 15ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the extracellular space. Protocol available in COMET™ Panel Builder.
COL4A1 in Human Liver via seqIF™ stainingon COMET™
COL4A1 was detected in immersion fixed paraffin-embedded sections of human Liver using Mouse Anti-Human COL4A1, Monoclonal Antibody (Catalog#MAB11758) at 15ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the extracellular space. Protocol available in COMET™ Panel Builder.
COL4A1 in Human Liver Carcinoma via seqIF™staining on COMET™
COL4A1 was detected in immersion fixed paraffin-embedded sections of human liver carcinoma using Mouse Anti-Human COL4A1, Monoclonal Antibody (Catalog#MAB11758) at 15ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH).Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the extracellular space. Protocol available in COMET™ Panel Builder.
Applications for Human Collagen IV alpha1 Antibody
Application
Recommended Usage
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney, human liver cancer and human liver
Sample: Immersion fixed paraffin-embedded sections of human kidney, human liver cancer and human liver
Multiplex Immunofluorescence
15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney, liver and liver carcinoma
Sample: Immersion fixed paraffin-embedded sections of human kidney, liver and liver carcinoma
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Collagen IV alpha 1
COL4A1 (collagen IV alpha1) is a 185 kDa member of the type IV collagen family. It is a secreted glycoprotein that is expressed by multiple cell types, including fibroblasts, keratinocytes and endothelial cells. Two COL4A1 molecules interact with a 170 kDa alpha2 chain to form a collagen IV triple helix. This helix further interacts with other helices to generate covalent oligomers that form a scaffold in the basement membrane. Mature human COL4A1 is 1642 amino acids (aa) in length. It has an N-terminal “7S” proregion (aa 28-172), a central collagenous domain that contains multiple Gly-based repeats (aa 173-1440), and a C-terminal domain that is proteolytically cleaved to generate a 25-28 kDa NC1 globular segment that has potent antiangiogenic activity (aa 1441-1669). Multiple splice forms exist. One shows a deletion of aa 499-849, a second shows a seven aa substitution for aa 513-1669, and a third shows a seven aa substitution for aa 958-1669. Over aa 1441-1669, human COL4A1 is 97% aa identical to mouse COL4A1.
Alternate Names
Arresten, COL4A1
Gene Symbol
COL4A1
UniProt
Additional Collagen IV alpha 1 Products
Product Documents for Human Collagen IV alpha1 Antibody
Product Specific Notices for Human Collagen IV alpha1 Antibody
For research use only
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