Human CLEC17A APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB101471A

R&D Systems, part of Bio-Techne
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FAB101471A-100
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 1064719

View all CLEC17A Antibodies »

Product Specifications

Immunogen

CHO-derived human CLEC17A
Lys194-Cys378
Accession # Q6ZS10

Specificity

Detects human CLEC17A in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CLEC17A APC-conjugated Antibody

Detection of CLEC17A in HEK293 cells by Flow Cytometry.

HEK293 cells transfected with Human CLEC17A and eGFP were stained with either (A) Mouse Anti-Human CLEC17A APC-conjugated Monoclonal Antibody (Catalog # FAB101471A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CLEC17A APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 cells transfected with Human CLEC17A and eGFP vs. Mouse IgG1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CLEC17A

C-type lectin domain family 17, member A (CLEC17A), also known as Prolectin, is type II transmembrane protein that is expressed mainly on dividing B cells found in the germinal centers of secondary lymphoid organs, including lymph nodes, tonsils, stomach, intestine, appendix and spleen (1, 2). CLEC17A binds preferentially to epithelial rather than to mesenchymal cells, and it behaves as a cell adhesion molecule for epithelial cells (2).  It has high specificity towards mannose and was found to form disulfide-linked oligomers (1, 3). Human CLEC17A is synthesized as a 378 amino acid (aa) protein that includes a 172 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 185 aa extracellular domain (ECD). Within the ECD, human CLEC17A shares 84% aa sequence identity with canine CLEC17A. C-type lectins are Ca2+-depending sugar-binding proteins that are involved in several immune-related and other physiological functions. Presently, 17 groups within the C-type lectin superfamily have been recognized (4), with more C-type lectins being constantly discovered based on the presence of a conserved 115-130 amino acid domain along their sequences - the C-type carbohydrate recognition domain (CRD). However, for most of the recently identified C-type lectins, their interactions with carbohydrates, intracellular functions and molecular mechanisms still remain unclear (3).

References

  1. Graham, S. A. et al. (2009) J. Biol. Chem. 284:18537.
  2. Breiman, A. et al. (2016) Oncotarget 7:14064.
  3. Koh, G. et al. (2011) BMC Bioinformatics 12:S5.
  4. Zelensky AN. et al. (2005) FEBS Journal 272:6179.

Long Name

C-type Lectin Domain Family 17, Member A

Alternate Names

Prolectin

Entrez Gene IDs

388512 (Human)

Gene Symbol

CLEC17A

UniProt

Q6ZS10

Additional CLEC17A Products

Product Documents for Human CLEC17A APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CLEC17A APC-conjugated Antibody

For research use only

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