Detects human Claudin‑4. Stains human Claudin-4 transfectants but not irrelevant transfectants. Recognizes an epitope in the first extracellular loop (aa Met29-Arg81).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human Claudin-4 Antibody

Detection of Claudin‑4 in PC‑3 Human Cell Line by Flow Cytometry.

PC-3 human prostate cancer cell line was stained with Mouse Anti-Human Claudin-4 Monoclonal Antibody (Catalog # MAB4219, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Claudin-4 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: PC-3 human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-4

Claudin-4 is a multipass membrane protein in the claudin family of tight junction proteins. Claudin-4 is a 209 amino acid molecule with four transmembrane segments. It is preferentially expressed in intestinal mucosa epithelium and is involved in epithelial tissue development. It is also up‑regulated in several carcinomas. Phosphorylation of Claudin-4 regulates paracellular epithelial permeability. Human Claudin-4 shares 83% aa sequence identity with mouse and rat Claudin-4.

Alternate Names

Claudin4, CLDN4, CPE-R, CPETR1, WBSCR8

Entrez Gene IDs

1364 (Human); 12740 (Mouse); 304407 (Rat)

Gene Symbol

CLDN4

UniProt

NP_001296

Additional Claudin-4 Products

Product Documents for Human Claudin-4 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Claudin-4 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Claudin-4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4219

Key Product Details

Species Reactivity

Validated:

Cited:

Applications