Human CDK5 Activator 1 Alexa Fluor® 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB7357G
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 739304
Product Specifications
Immunogen
E. coli-derived recombinant human CDK5 Activator 1
Ser8-Phe98
Accession # Q15078
Ser8-Phe98
Accession # Q15078
Specificity
Detects human CDK5 Activator 1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CDK5 Activator 2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human CDK5 Activator 1 Alexa Fluor® 488-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: CDK5 Activator 1
Cyclin dependent kinase 5 regulatory subunit 1 (CDK5R1) is a 35 kDa protein that associates with and activates CDK5. Its expression is restricted to neurons and is required for normal brain development. CDK5R1 can be cleaved by calpain at Phe98-Ala99 which liberates a C-terminal fragment known as p25. p25 induces prolonged activation of CDK5 and broadens the substrate specificity of CDK5 to include tau which is aberrantly phosphorylated in Alzheimer's disease. Full length CDK5R1 is myristoylated at Gly2 and is preferentially localized to membranes at the cell periphery. p25 lacks this modification and is enriched in the nucleus and perinuclear regions. p25 accumulates in the brains of Alzheimer's patients where it promotes neuronal apoptosis and cytoskeletal abnormalities. Within aa 100-307, human CDK5R1 shares 99% aa sequence identity with mouse and rat CDK5R1.
Long Name
Cyclin-dependent Kinase 5, Regulatory Subunit 1 (p35)
Alternate Names
CDK5P35, CDK5R1, NCK5A, Neuronal CDK5 Activator, p35nck5a, TPKII Regulatory Subunit
Gene Symbol
CDK5R1
UniProt
Additional CDK5 Activator 1 Products
Product Specific Notices for Human CDK5 Activator 1 Alexa Fluor® 488-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
Loading...
Loading...
Loading...