Human CD96 v2 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB6199A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 628211
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD96
Val22-Asp501
Accession # NP_005807
Val22-Asp501
Accession # NP_005807
Specificity
Detects human CD96v2 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD96 v2 APC-conjugated Antibody
Detection of CD96v2 in Human PBMC Lymphocytes by Flow Cytometry.
Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human CD3e Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB100F) and either (A) Mouse Anti-Human CD96v2 APC-conjugated Monoclonal Antibody (Catalog # FAB6199A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.Applications for Human CD96 v2 APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood mononuclear cell (PBMC) lymphocytes
Sample: Human peripheral blood mononuclear cell (PBMC) lymphocytes
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: CD96
CD96 (also known as Tactile) is a 160 kDa type I transmembrane glycoprotein that contains three Ig-like domains in its extracellular region. It is expressed on CD4+ and CD8+ T cells, NK cells, and select B cells. CD96 binds to CD155/PVR and participates in NK cell-mediated lysis of CD155+ target cells. Alternate splicing generates a short variant (CD96v2) which lacks 16 amino acids (aa) within the second Ig-like domain. CD96v2 is the predominant isoform in many cell types and exhibits even greater binding affinity with CD155 than does full length CD96. A soluble form of CD96 circulates in the serum. Over aa 22-501, human CD96v2 shares 55% aa sequence identity with mouse and rat CD96.
Alternate Names
CD96, TACTILE
Gene Symbol
CD96
UniProt
Additional CD96 Products
Product Documents for Human CD96 v2 APC-conjugated Antibody
Product Specific Notices for Human CD96 v2 APC-conjugated Antibody
For research use only
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