Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # HB15e

Product Summary for Human CD83 PE‑conjugated Antibody

Immunogen

COS-7 African green monkey SV40 transformed kidney fibroblast-like cells transfected with human CD83

Specificity

Detects human CD83 in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CD83 PE‑conjugated Antibody

Detection of CD83 antibody in CD14+ Monocyte-derived Dendritic Cells antibody by Flow Cytometry.

Detection of CD83 in CD14+ Monocyte-derived Dendritic Cells by Flow Cytometry.

CD14+ monocyte-derived dendritic cells were stained with Mouse Anti-Human CD83 PE-conjugated Monoclonal Antibody (Catalog # FAB1774P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of CD83 in Daudi cells by Flow Cytometry.

Daudi cells were stained with Mouse Anti-Human CD83 PE‑conjugated Monoclonal Antibody (Catalog # FAB1774P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human CD83 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: CD14+ monocyte-derived dendritic cells and Daudi human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 3 publications using FAB1774P in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD83

Human CD83 is a 40‑50 kDa member of the Siglec (or Sialic-acid-binding Immunoglobulin-like Lectin) family of transmembrane proteins (1, 2, 3). CD83 is synthesized as a type I transmembrane glycoprotein that contains a 125 amino acid (aa) extracellular region, a 22 aa transmembrane segment, and 39 aa cytoplasmic domain. It contains one V type Ig-like domain in the extracellular region with no inhibitory cytoplasmic motif(s). Although in vitro studies suggest CD83 may form membrane-bound covalent homodimers, in vivo this does not appear to be the case (1, 4). In the extracellular region, mouse and human CD83 are 66% aa identical (1, 2, 4, 5). Relative to human, mouse CD83 is 11 aa shorter in its extracellular domain and is expressed as a 30‑35 kDa protein (1, 4, 5). Human CD83 is active in the mouse system (4). One alternate splice form has been reported. This leads to a small monomeric soluble form of 74 aa that includes aa 20‑52 and aa 164‑205 (6, 7). In human, proteolytic cleavage and solubilization of CD83 has also been suggested, and this could lead to dimeric circulating CD83 (4, 6). CD83 is a primary marker for dendritic cells (3, 6, 8). It is also found on B cells (6, 9), neutrophils (10), monocytes and macrophages (11). Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on target cells (12). Membrane CD83 appears to promote T cell proliferation, particularly of CD8+ cytotoxic T cells (13, 14). Soluble CD83, however, appears to be immunosuppressive and blocks T cell activation (15, 16). On monocytes, CD83 is suggested to drive monocytes into a fibrocyte phenotype (13). A lack of membrane-expressed CD83 leads to an unusual IL-4/IL-10 producing CD4+ T cell phenotype (17).

References

  1. Zhou, L-J. et al. (1992) J. Immunol. 149:735.
  2. Kozlow, E.J. et al. (1993) Blood 81:454.
  3. Fujimoto, Y and T.F. Tedder (2006) J. Med. Dent. Sci. 53:85.
  4. Lechmann, M. et al. (2005) Biochem. Biophys. Res. Commun. 329:132.
  5. Berchtold, S. et. al. (1999) FEBS Lett. 461:211.
  6. Hock, B.D. et al. (2001) Int. Immunol. 13:959.
  7. Dudziak, D. et al. (2005) J. Immunol. 174:6672.
  8. Velten, F.W. et al. (2007) Mol. Immunol. 44:1544.
  9. Cramer, S.O. et al. (2000) Int. Immunol. 12:1347.
  10. Yamashiro, S. et al. (2000) Blood 96:3958.
  11. Cao, W. et al. (2005) Biochem. J. 385:85.
  12. Scholler, N. et al. (2001) J. Immunol. 166:3865.
  13. Scholler, N. et al. (2002) J. Immunol. 168:2599.
  14. Hirano, N. et al. (2006) Blood 107:1528.
  15. Kotzor, N. et al. (2004) Immunobiology 209:129.
  16. Zinser, E. et al. (2006) Immunobiology 211:449.
  17. Garcia-Martinez, L.F. et al. (2004) J. Immunol. 173:2995.

Alternate Names

BL11, CD83, HB15

Entrez Gene IDs

9308 (Human); 12522 (Mouse)

Gene Symbol

CD83

Additional CD83 Products

Product Documents for Human CD83 PE‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human CD83 PE‑conjugated Antibody

For research use only

Loading...