Human CD58/LFA-3 Antibody

Name: Human CD58/LFA-3 Antibody
Brand: R&D Systems
SKU: AF1689

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1689

Citations (2)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human CD58/LFA‑3 Phe29-Arg215 Accession # P19256
Applications:	Block/Neutralize, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human CD58/LFA‑3 in direct ELISAs and Western blots.

View Full Technical Details for Human CD58/LFA-3 Antibody

Applications for Human CD58/LFA-3 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human CD58/LFA‑3 (Catalog # 1689-CD)
Immunohistochemistry	5-15 µg/mL	See below

Item	Value
Neutralization	Measured by its ability to neutralize CD58/LFA‑3-induced proliferation in the human CD3⁺ T cells. Bierer, B.E. et al. (1988) J. Immunol. 140:3358. The Neutralization Dose (ND₅₀) is typically 3-12 µg/mL in the presence of 0.2 µg/well Recombinant Human CD58/LFA‑3 and 2 ng/well Mouse Anti-Human CD3 Monoclonal Antibody OKT3.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CD58/LFA-3 Antibody

	Immunohistochemistry CD58/LFA‑3 in Human Tonsil. CD58/LFA‑3 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Goat Anti-Human CD58/LFA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1689) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of lymphocytes in lymphatic nodules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Neutralization Cell Proliferation Induced by CD58/LFA‑3 and Neutral-ization by Human CD58/LFA‑3 Antibody. Recombinant Human CD58/LFA-3 (Catalog # 1689-CD) stimulates proliferation in the human CD3⁺T cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CD58/LFA-3 (0.2 µg/well) is neutralized (green line) by increasing concentrations of Goat Anti-Human CD58/LFA-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1689). The ND₅₀ is typically 3-12 µg/mL in the presence of Mouse Anti-Human CD3 Monoclonal Antibody OKT3 (2 ng/well).

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CD58/LFA-3

T cells require a signal induced by the engagement of the T cell receptor and a “co-stimulatory” signal(s) through distinct T cell surface molecules for optimal T cell expansion and activation. Many cell-bound receptor-ligand pairs have now been shown to be involved in T cell co-stimulation including CD58/CD2 in humans and CD48/CD2 in mice and rats. CD58, also known as lymphocyte function-associated antigen (LFA-3), is a 210 amino acid protein that belongs to the CD2 family of the immunoglobulin superfamily (1). CD58 is widely expressed on hematopoietic and non-hematopoietic human tissue and has been found on leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts of human origin (2). No mouse or rat homolog of CD58 has as of yet been identified. CD58 has only one known ligand, CD2. CD2 is expressed on T cells, NK cells and dendritic cells (2-4). CD2 ligation by CD58 has been shown to mediate T cell adhesion, T cell activation, T cell cytokine production, and T cell and NK cells cytotoxic activity (1, 3, 5, 6). In dendritic cells, CD2 engagement increases MHC Class II, CD40, CD80, CD86, CD58, and CCR7 and induces IL-1 beta and IL-12 cytokine secretion (4).

Item	Value
References	Davis, S.J. and P.A. van der Merwe (1996) Immunol. Today 17:177. Smith, M.E. and J.A. Thomas (1990) J. Clin. Pathol. 43:893. Bolhuis, R.L. Roozemond, R.C. and R.J. van de Griend (1986) J. Immunol. 136:3939. Crawford, K. et al. (2003) Blood 102:1745. Kanner, S.B. et al. (1992) J. Immunol. 148:2023. Bullens, M.A. et al. (2001) International Immunol. 12:181.
Entrez Gene IDs	965 (Human); 102123839 (Cynomolgus Monkey); 710732 (Rhesus Macaque)
Alternate Names	Ag3, CD58, CD58 antigen, CD58 antigen, (lymphocyte function-associated antigen 3), CD58 molecule, FLJ23181, FLJ43722, LFA-3, LFA3, LFA3ag3, Surface glycoprotein LFA-3, lymphocyte function-associated antigen 3

Citations for Human CD58/LFA-3 Antibody (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human CD58/LFA-3 Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC

Y Zhang et al. (2021), Upregulated CD58 is associated with clinicopathological characteristics and poor prognosis of patients with pancreatic ductal adenocarcinoma Cancer Cell International, 21(1):327.
PMID: 34193136
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

M Kim et al. (2018), Novel natural killer cell-mediated cancer immunotherapeutic activity of anisomycin against hepatocellular carcinoma cells Sci Rep, 8(1):10668.
PMID: 30006566

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Human CD58/LFA-3 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human CD58/LFA-3 Antibody

Scientific Data Examples for Human CD58/LFA-3 Antibody

Immunohistochemistry

Neutralization

Formulation, Preparation and Storage

Reconstitution Calculator

Background: CD58/LFA-3

Citations for Human CD58/LFA-3 Antibody (2)

Product Documents for Human CD58/LFA-3 Antibody

Product Datasheets

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