Human CD4 Antibody

Name: Human CD4 Antibody
Brand: R&D Systems
SKU: AF-379-NA
Rating: 4 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF-379-NA

Citations (13)

(2)

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Key Product Details

Species Reactivity:	Human
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD4 Lys26-Trp390 Accession # P01730
Applications:	Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human CD4 in direct ELISAs and Western blots.

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Applications for Human CD4 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human CD4 (Catalog # 514-CD)
Immunohistochemistry	1-15 µg/mL	Immersion fixed paraffin-embedded sections of human tonsil tissue
Immunocytochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human CD4 Antibody

	Immunocytochemistry CD4 in Human T Cells. CD4 was detected in immersion fixed human T cells using 2 µg/mL Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑379‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunocytochemistry CD4 in Human PBMCs. CD4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
	Immunohistochemistry CD4 in Human Tonsil Tissue. CD4 was detected in immersion fixed paraffin-embedded sections of human tonsil tissue using Goat Anti-Human CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-379-NA) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents protocol.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CD4

CD4 is an approximately 55 kDa type I membrane glycoprotein that is expressed predominantly on most thymocytes and a subset of mature T lymphocytes. In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells. Human CD4 cDNA encodes a 458 amino acid (aa) residue precursor protein with a 25 aa residue signal peptide, a 371 aa residue extracellular region containing four immunoglobulin homology domains, a 24 aa residue transmembrane domain and a 38 aa residue cytoplasmic domain. CD4 is a coreceptor required for T cell recognition of antigens that are presented by class II major histocompatibility complexes. CD4 has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.

Item	Value
References	Capon, D.I. et al. (1991) Annu. Rev. Immunol. 9:649.
Entrez Gene IDs	920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 101864991 (Cynomolgus Monkey); 493775 (Feline)
Alternate Names	CD4, CD4 antigen, CD4 antigen (p55), CD4 molecule, CD4 receptor, CD4mut, CD_antigen: CD4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Citations for Human CD4 Antibody (13)

Showing 1 - 10 of 13 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human CD4 Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

K Sangare et al. (2022-01-01), E4orf1 Suppresses E1B-Deleted Adenovirus Vaccine-Induced Immune Responses Vaccines, 10(2).
PMID: 35214753
Species: Human
Sample Types: Whole Tissue
Applications: IHC

R Haddad et al. (2022-01-01), Tumor Lymphocyte Infiltration Is Correlated with a Favorable Tumor Regression Grade after Neoadjuvant Treatment for Esophageal Adenocarcinoma Journal of personalized medicine, 12(4).
PMID: 35455743
Species: Human
Sample Types: Whole Tissue
Applications: IHC

BJ Mehrara et al. (2021-01-01), Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema Biology, 10(9).
PMID: 34571811
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

JP Sutherland et al. (2019-01-01), Persistence of a T Cell Infiltrate in Human Ganglia Years After Herpes Zoster and During Post-herpetic Neuralgia Front Microbiol, 10(0):2117.
PMID: 31572325
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Reissfelder C et al. (2015-01-01), Tumor-specific cytotoxic T lymphocyte activity determines colorectal cancer patient prognosis. J Clin Invest, 125(2):739-51.
PMID: 25562322
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Del Corno M et al. (2014-01-01), HIV-1 gp120 activates the STAT3/interleukin-6 axis in primary human monocyte-derived dendritic cells. J Virol, 88(19):11045-55.
PMID: 25008924
Species: Human
Sample Types: Whole Cells
Applications: ICC

Suzuki et al. (2014-01-01), Slow turnover of HIV-1 receptors on quiescent CD4+ T cells causes prolonged surface retention of gp120 immune complexes in vivo. PLoS ONE, 9(2):e86479.
PMID: 24516533
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Chan Y et al. (2013-01-01), Patients with cystic fibrosis have inducible IL-17+IL-22+ memory cells in lung draining lymph nodes. J Allergy Clin Immunol, 131(4):1117-29, 1129.
PMID: 22795370
Species: Human
Sample Types: Whole Cells
Applications: ICC

Swaminathan G et al. (2012-01-01), A role for microRNA-155 modulation in the anti-HIV-1 effects of Toll-like receptor 3 stimulation in macrophages. PLoS Pathog, 8(9):e1002937.
PMID: 23028330
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Fasth AE et al. (2009-01-01), T cell infiltrates in the muscles of patients with dermatomyositis and polymyositis are dominated by CD28null T cells. J. Immunol., 183(7):4792-9.
PMID: 19752224
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Englund P et al. (2007-01-01), Restricted T cell receptor BV gene usage in the lungs and muscles of patients with idiopathic inflammatory myopathies. Arthritis Rheum., 56(1):372-83.
PMID: 17195241
Species: Human
Sample Types: Whole Cells
Applications: ICC

Bjerke SL et al. (2006-01-01), Roles for herpes simplex virus type 1 UL34 and US3 proteins in disrupting the nuclear lamina during herpes simplex virus type 1 egress. Virology, 347(2):261-76.
PMID: 16427676
Species: Human
Sample Types: Whole Cells
Applications: ICC

Wang B et al. (2006-01-01), An effective cancer vaccine modality: lentiviral modification of dendritic cells expressing multiple cancer-specific antigens. Vaccine, 24(17):3477-89.
PMID: 16530303

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Customer Reviews for Human CD4 Antibody (2)

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Human CD4 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Colon cancer tissue

Species: Human

Verified Customer | Posted 04/06/2021
Human CD4 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Tonsil tissue

Species: Human and Human tonsil

Verified Customer | Posted 12/02/2019

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Human CD4 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human CD4 Antibody

Scientific Data Examples for Human CD4 Antibody

Immunocytochemistry

Immunocytochemistry

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: CD4

Citations for Human CD4 Antibody (13)

Customer Reviews for Human CD4 Antibody (2)

Customer Images

Human CD4 Antibody

Human CD4 Antibody

Product Documents for Human CD4 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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